ArticleScientific reports2016
The Type II Secreted Lipase/Esterase LesA is a Key Virulence Factor Required for Xylella fastidiosa Pathogenesis in Grapevines.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 110 citations in OpenAlex.
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- Toward a better understanding of the ecological roles of extracellular vesicles from plant-associated bacteria.Applied and environmental microbiology · 2025Review
- Functional Cargo in Membrane Vesicles From a Citrus Pathogen.Environmental microbiology reports · 2025Article
- Extracellular Vesicles From Xylella fastidiosa Carry sRNAs and Genomic Islands, Suggesting Roles in Recipient Cells.Journal of extracellular vesicles · 2025Article
- From insect endosymbiont to phloem colonizer: comparative genomics unveils the lifestyle transition of phytopathogenicmSystems · 2025Article
- The Role of Heat Shock Protein (Hsp) Chaperones in Environmental Stress Adaptation and Virulence of Plant Pathogenic Bacteria.International journal of molecular sciences · 2025Review
- Practical advice for extracellular vesicle isolation in plant-microbe interactions: Concerns, considerations, and conclusions.Journal of extracellular vesicles · 2024Article
- Comparative transcriptomics reveals a highly polymorphic Xanthomonas HrpG virulence regulon.BMC genomics · 2024Article
- Assessment of Fatty Acid and Oxylipin Profile of Resprouting Olive Trees Positive toPlants (Basel, Switzerland) · 2024Article
- Expected potential hemipteran vectors ofHeliyon · 2024Article
- Xylem-dwelling pathogen unaffected by local xylem vessel network properties in grapevines (Vitis spp.).Annals of botany · 2024Article
- The Exometabolome ofMetabolites · 2024Article
- Membrane vesicle engineering with "à la carte" bacterial-immunogenic molecules for organism-free plant vaccination.Microbial biotechnology · 2023Review
- Transcriptome and Secretome Analyses of EndophyteMicroorganisms · 2023Article
- Article
- Bacterial secretion system functions: evidence of interactions and downstream implications.Microbiology (Reading, England) · 2023Article
- Proteomic Profiling Reveals Distinct Bacterial Extracellular Vesicle Subpopulations with Possibly Unique Functionality.Applied and environmental microbiology · 2023Article
- The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic BacteriumMicroorganisms · 2022Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
11 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pierce's disease (PD) of grapevines is caused by Xylella fastidiosa (Xf), a xylem-limited gamma-proteobacterium that is responsible for several economically important crop diseases. The occlusion of xylem elements and interference with water transport by Xf and its associated biofilm have been posited as the main cause of PD symptom development; however, Xf virulence mechanisms have not been described. Analysis of the Xf secretome revealed a putative lipase/esterase (LesA) that was abundantly secreted in bacterial culture supernatant and was characterized as a protein ortholog of the cell wall-degrading enzyme LipA of Xanthomonas strains. LesA was secreted by Xf and associated with a biofilm filamentous network. Additional proteomic analysis revealed its abundant presence in outer membrane vesicles (OMVs). Accumulation of LesA in leaf regions associated positively with PD symptoms and inversely with bacterial titer. The lipase/esterase also elicited a hypersensitive response in grapevine. Xf lesA mutants were significantly deficient for virulence when mechanically inoculated into grapevines. We propose that Xf pathogenesis is caused by LesA secretion mediated by OMV cargos and that its release and accumulation in leaf margins leads to early stages of observed PD symptoms.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.