ArticleJournal of biophotonics2016
Visualising apoptosis in live zebrafish using fluorescence lifetime imaging with optical projection tomography to map FRET biosensor activity in space and time.
Article in Journal of biophotonics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Non-apoptotic caspase events and Atf3 expression underlie direct neuronal differentiation of adult neural stem cells.Development (Cambridge, England) · 2024Article
- Advancements in fluorescence lifetime imaging microscopy Instrumentation: Towards high speed and 3D.Current opinion in solid state & materials science · 2024Article
- Generation of a Transgenic Zebrafish Line for In Vivo Assessment of Hepatic Apoptosis.Pharmaceuticals (Basel, Switzerland) · 2021Article
- High resolution optical projection tomography platform for multispectral imaging of the mouse gut.Biomedical optics express · 2021Article
- Luminescence lifetime imaging of three-dimensional biological objects.Journal of cell science · 2021Article
- Drug Screening with Genetically Encoded Fluorescent Sensors: Today and Tomorrow.International journal of molecular sciences · 2020Review
- Three-dimensional bright-field microscopy with isotropic resolution based on multi-view acquisition and image fusion reconstruction.Scientific reports · 2020Article
- Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications.Journal of biomedical optics · 2020Review
- Highlights on selected microscopy techniques to study zebrafish developmental biology.Laboratory animal research · 2020Review
- Angular restriction fluorescence optical projection tomography to localize micrometastases in lymph nodes.Journal of biomedical optics · 2019Article
- OptiJ: Open-source optical projection tomography of large organ samples.Scientific reports · 2019Article
- Quantitative imaging of receptor-ligand engagement in intact live animals.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- A FRET-based fluorescent and colorimetric probe for the specific detection of picric acid.RSC advances · 2018Article
- A Genetically Encoded Biosensor Strategy for Quantifying Non-muscle Myosin II Phosphorylation Dynamics in Living Cells and Organisms.Cell reports · 2018Article
- Preparing Fresh Retinal Slices from Adult Zebrafish for Ex Vivo Imaging Experiments.Journal of visualized experiments : JoVE · 2018Article
- Utilizing Zebrafish Visual Behaviors in Drug Screening for Retinal Degeneration.International journal of molecular sciences · 2017Review
- Optimal Anesthetic Regime for Motionless Three-Dimensional Image Acquisition During Longitudinal Studies of Adult Nonpigmented Zebrafish.Zebrafish · 2017Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 1 country.
Funding
Abstract
Fluorescence lifetime imaging (FLIM) combined with optical projection tomography (OPT) has the potential to map Förster resonant energy transfer (FRET) readouts in space and time in intact transparent or near transparent live organisms such as zebrafish larvae, thereby providing a means to visualise cell signalling processes in their physiological context. Here the first application of FLIM OPT to read out biological function in live transgenic zebrafish larvae using a genetically expressed FRET biosensor is reported. Apoptosis, or programmed cell death, is mapped in 3-D by imaging the activity of a FRET biosensor that is cleaved by Caspase 3, which is a key effector of apoptosis. Although apoptosis is a naturally occurring process during development, it can also be triggered in a variety of ways, including through gamma irradiation. FLIM OPT is shown here to enable apoptosis to be monitored over time, in live zebrafish larvae via changes in Caspase 3 activation following gamma irradiation at 24 hours post fertilisation. Significant apoptosis was observed at 3.5 hours post irradiation, predominantly in the head region.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.