ArticleBiomolecular detection and quantification2015
Assessing the performance of the Oxford Nanopore Technologies MinION.
Article in Biomolecular detection and quantification, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 257 papers, 1 of them a synthesis that pooled it.
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Who cites it
257 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Technical feasibility of a long read, fourth generation sequencing platform in diagnostic profiling of clinical routine samples: a proof-of-concept study.Pathologica · 2026Article
- Whole-genome sequence of a female Loa loa adult worm from Cameroon.BMC research notes · 2026Article
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- NanoPrePro: a fully equipped, fast, and memory-efficient preprocessor for nanopore transcriptomic sequencing.Briefings in bioinformatics · 2026Article
- TNRSim - a simulator of truncated oxford nanopore direct RNA long reads for isoform analysis.Frontiers in bioinformatics · 2026Article
- Shotgun Metagenomic Analysis of Microbial Community Dynamics in Wastewater Treatment Through Constructed Wetlands.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Targeted long-read nanopore sequencing as a complementary approach for detecting STRC variants and distinguishing the STRCP1 pseudogene.Scientific reports · 2025Article
- NaMeco - Nanopore full-length 16S rRNA gene reads clustering and annotation.BMC genomics · 2025Article
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- Impact of RNA extraction on respiratory microbiome analysis using third-generation sequencing.BMC genomics · 2025Article
- Constrained coding for error mitigation in nanopore-based DNA data storage.Scientific reports · 2025Article
- Development of a Rapid, Culture-Free, Universal Microbial Identification System Using Internal Transcribed Spacer Targeting Primers.The Journal of infectious diseases · 2025Article
- Article
197 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
Abstract
The Oxford Nanopore Technologies (ONT) MinION is a new sequencing technology that potentially offers read lengths of tens of kilobases (kb) limited only by the length of DNA molecules presented to it. The device has a low capital cost, is by far the most portable DNA sequencer available, and can produce data in real-time. It has numerous prospective applications including improving genome sequence assemblies and resolution of repeat-rich regions. Before such a technology is widely adopted, it is important to assess its performance and limitations in respect of throughput and accuracy. In this study we assessed the performance of the MinION by re-sequencing three bacterial genomes, with very different nucleotide compositions ranging from 28.6% to 70.7%; the high G + C strain was underrepresented in the sequencing reads. We estimate the error rate of the MinION (after base calling) to be 38.2%. Mean and median read lengths were 2 kb and 1 kb respectively, while the longest single read was 98 kb. The whole length of a 5 kb rRNA operon was covered by a single read. As the first nanopore-based single molecule sequencer available to researchers, the MinION is an exciting prospect; however, the current error rate limits its ability to compete with existing sequencing technologies, though we do show that MinION sequence reads can enhance contiguity of de novo assembly when used in conjunction with Illumina MiSeq data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.