ArticlePloS one2016
PpASCL, the Physcomitrella patens Anther-Specific Chalcone Synthase-Like Enzyme Implicated in Sporopollenin Biosynthesis, Is Needed for Integrity of the Moss Spore Wall and Spore Viability.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Moss BRCA2 lacking the canonical DNA-binding domain promotes homologous recombination and binds to DNA.Nucleic acids research · 2025Article
- The chloroplast-located HKT transporter plays an important role in fertilization and development in Physcomitrium patens.The Plant journal : for cell and molecular biology · 2025Article
- CRISPR/Cas9-based genome editing of 14 lipid metabolic genes reveals a sporopollenin metabolon ZmPKSB-ZmTKPR1-1/-2 required for pollen exine formation in maize.Plant biotechnology journal · 2024Article
- Sporogenesis inFrontiers in cell and developmental biology · 2023Article
- MS1/MMD1 homologues in the moss Physcomitrium patens are required for male and female gametogenesis.The New phytologist · 2022Article
- Dependence on clade II bHLH transcription factors for nursing of haploid products by tapetal-like cells is conserved between moss sporangia and angiosperm anthers.The New phytologist · 2022Article
- The Toughest Material in the Plant Kingdom: An Update on Sporopollenin.Frontiers in plant science · 2021Review
- Article
- The MossThe Plant cell · 2020Review
- Article
- Studies of a rice sterile mutant sstl from the TRIM collection.Botanical studies · 2019Article
- OsPKS2 is required for rice male fertility by participating in pollen wall formation.Plant cell reports · 2018Article
- Article
- OsLAP6/OsPKS1, an orthologue of Arabidopsis PKSA/LAP6, is critical for proper pollen exine formation.Rice (New York, N.Y.) · 2017Article
- Development and genetics in the evolution of land plant body plans.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
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Authors and funding
8 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Sporopollenin is the main constituent of the exine layer of spore and pollen walls. The anther-specific chalcone synthase-like (ASCL) enzyme of Physcomitrella patens, PpASCL, has previously been implicated in the biosynthesis of sporopollenin, the main constituent of exine and perine, the two outermost layers of the moss spore cell wall. We made targeted knockouts of the corresponding gene, PpASCL, and phenotypically characterized ascl sporophytes and spores at different developmental stages. Ascl plants developed normally until late in sporophytic development, when the spores produced were structurally aberrant and inviable. The development of the ascl spore cell wall appeared to be arrested early in microspore development, resulting in small, collapsed spores with altered surface morphology. The typical stratification of the spore cell wall was absent with only an abnormal perine recognisable above an amorphous layer possibly representing remnants of compromised intine and/or exine. Equivalent resistance of the spore walls of ascl mutants and the control strain to acetolysis suggests the presence of chemically inert, defective sporopollenin in the mutants. Anatomical abnormalities of late-stage ascl sporophytes include a persistent large columella and an air space incompletely filled with spores. Our results indicate that the evolutionarily conserved PpASCL gene is needed for proper construction of the spore wall and for normal maturation and viability of moss spores.
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