Evidence map›Paper›PMID 26752561›Full record

ArticlePloS one2016

Proteomics Based Identification of Proteins with Deregulated Expression in B Cell Lymphomas.

Rui Wu, Marcel Nijland, Bea Rutgers, Rianne Veenstra, Myra Langendonk, Lotte E van der Meeren, Philip M Kluin, Guanwu Li, Arjan Diepstra, Jen-Fu Chiu and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Article
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Rui WuDepartment of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Marcel NijlandDepartment of Hematology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Bea RutgersDepartment of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Rianne VeenstraDepartment of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Myra LangendonkDepartment of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Lotte E van der MeerenDepartment of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Philip M KluinDepartment of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Guanwu LiDepartment of Biochemistry, Open laboratory for Tumor Molecular Biology, Shantou University Medical College, Shantou, China.
Arjan DiepstraDepartment of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Jen-Fu ChiuDepartment of Biochemistry, Open laboratory for Tumor Molecular Biology, Shantou University Medical College, Shantou, China.
Anke van den BergDepartment of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
Lydia VisserDepartment of Pathology and Medical Biology, University of Groningen and University Medical Center Groningen, Groningen, The Netherlands.
University of Groningen · NLUniversity Medical Center Groningen · NLShantou University · CNShantou University Medical College · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Follicular lymphoma and diffuse large B cell lymphomas comprise the main entities of adult B cell malignancies. Although multiple disease driving gene aberrations have been identified by gene expression and genomic studies, only a few studies focused at the protein level. We applied 2 dimensional gel electrophoresis to compare seven GC B cell non Hodgkin lymphoma (NHL) cell lines with a lymphoblastoid cell line (LCL). An average of 130 spots were at least two folds different in intensity between NHL cell lines and the LCL. We selected approximately 38 protein spots per NHL cell line and linked them to 145 unique spots based on the location in the gel. 34 spots that were found altered in at least three NHL cell lines when compared to LCL, were submitted for LC-MS/MS. This resulted in 28 unique proteins, a substantial proportion of these proteins were involved in cell motility and cell metabolism. Loss of expression of B2M, and gain of expression of PRDX1 and PPIA was confirmed in the cell lines and primary lymphoma tissue. Moreover, inhibition of PPIA with cyclosporine A blocked cell growth of the cell lines, the effect size was associated with the PPIA expression levels. In conclusion, we identified multiple differentially expressed proteins by 2-D proteomics, and showed that some of these proteins might play a role in the pathogenesis of NHL.

Indexed as

Gene Expression Regulation, NeoplasticCell Line, TumorChromatography, LiquidCyclosporineHumansImmunohistochemistryLymphoma, B-CellLymphoma, Non-HodgkinNeoplasm ProteinsProteomeProteomicsReproducibility of ResultsTandem Mass SpectrometryCyclosporineNeoplasm ProteinsProteome

Identifiers

PMID26752561
PMCPMC4708982
OpenAlexW2236880457

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.