ArticlePloS one2016
De Novo Transcriptome and Expression Profile Analysis to Reveal Genes and Pathways Potentially Involved in Cantharidin Biosynthesis in the Blister Beetle Mylabris cichorii.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 14 citations in OpenAlex.
- Identification of the key regulatory network and genes highly associated with the synthesis of cantharidin in Epicauta chinensis by transcriptome and metabolome analyses.Insect science · 2026Article
- Chemosensory System Decoding: Transcriptome-Wide Identification and Expression Profiling of Olfactory Genes inEcology and evolution · 2025Article
- Genome-Wide Profiling of P450 Gene Expression Reveals Caste-Specific and Developmental Patterns inInternational journal of molecular sciences · 2025Article
- Natural Occurring Terpene Cyclic Anhydrides: Biosynthetic Origin and Biological Activities.Biomolecules · 2024Review
- Functional studies of McSTE24, McCYP305a1, and McJHEH, three essential genes act in cantharidin biosynthesis in the blister beetle (Coleoptera: Meloidae).Journal of insect science (Online) · 2024Article
- Investigation of sex expression profiles and the cantharidin biosynthesis genes in two blister beetles.PloS one · 2023Article
- A minireview of the medicinal and edible insects from the traditional Chinese medicine (TCM).Frontiers in pharmacology · 2023Review
- Article
- Transcriptome Sequencing of the Striped Cucumber Beetle,Biotech (Basel (Switzerland)) · 2020Article
- Diversity of Insect Sesquiterpenoid Regulation.Frontiers in genetics · 2020Review
- Draft Genome of a Blister BeetleFrontiers in genetics · 2019Article
- Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dried body of Mylabris cichorii is well-known Chinese traditional medicine. The sesquiterpenoid cantharidin, which is secreted mostly by adult male beetles, has recently been used as an anti-cancer drug. However, little is known about the mechanisms of cantharidin biosynthesis. Furthermore, there is currently no genomic or transcriptomic information for M. cichorii. In this study, we performed de novo assembly transcriptome of M. cichorii using the Illumina Hiseq2000. A single run produced 9.19 Gb of clean nucleotides comprising 29,247 sequences, including 23,739 annotated sequences (about 81%). We also constructed two expression profile libraries (20-25 day-old adult males and 20-25 day-old adult females) and discovered 2,465 significantly differentially-expressed genes. Putative genes and pathways involved in the biosynthesis of cantharidin were then characterized. We also found that cantharidin biosynthesis in M. cichorii might only occur via the mevalonate (MVA) pathway, not via the methylerythritol 4-phosphate/deoxyxylulose 5-phosphate (MEP/DOXP) pathway or a mixture of these. Besides, we considered that cantharidin biosynthesis might be related to the juvenile hormone (JH) biosynthesis or degradation. The results of transcriptome and expression profiling analysis provide a comprehensive sequence resource for M. cichorii that could facilitate the in-depth study of candidate genes and pathways involved in cantharidin biosynthesis, and may thus help to improve our understanding of the mechanisms of cantharidin biosynthesis in blister beetles.
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