Evidence map›Paper›PMID 26752140›Full record

ArticleBone marrow transplantation2016

Low-dose irradiation prior to bone marrow transplantation results in ATM activation and increased lethality in Atm-deficient mice.

J Pietzner, B M Merscher, P C Baer, R P Duecker, O Eickmeier, D Fußbroich, P Bader, D Del Turco, R Henschler, S Zielen and 1 more

Erratum issuedOpen access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Bone marrow transplantation, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Trial
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

J PietznerDivision of Allergy, Pulmonology, and Cystic Fibrosis, Department for Children and Adolescents, Children's Hospital, Goethe University, Frankfurt, Germany.
B M MerscherDivision of Allergy, Pulmonology, and Cystic Fibrosis, Department for Children and Adolescents, Children's Hospital, Goethe University, Frankfurt, Germany.
P C BaerDepartment of Internal Medicine, Division of Nephrology, Goethe University, Frankfurt, Germany.
R P DueckerDivision of Allergy, Pulmonology, and Cystic Fibrosis, Department for Children and Adolescents, Children's Hospital, Goethe University, Frankfurt, Germany.
O EickmeierDivision of Allergy, Pulmonology, and Cystic Fibrosis, Department for Children and Adolescents, Children's Hospital, Goethe University, Frankfurt, Germany.
D FußbroichDivision of Allergy, Pulmonology, and Cystic Fibrosis, Department for Children and Adolescents, Children's Hospital, Goethe University, Frankfurt, Germany.
P BaderDivision for Stem Cell Transplantation and Immunology, Department for Children and Adolescents, Goethe University, Frankfurt, Germany.
D Del TurcoInstitute of Clinical Neuroanatomy, Neuroscience Center, Goethe University, Frankfurt, Germany.
R HenschlerInstitute of Transfusion Medicine and Immune Hematology, Blood Donation Service of the German Red Cross, Goethe University, Frankfurt, Germany.
S ZielenDivision of Allergy, Pulmonology, and Cystic Fibrosis, Department for Children and Adolescents, Children's Hospital, Goethe University, Frankfurt, Germany.
R SchubertDivision of Allergy, Pulmonology, and Cystic Fibrosis, Department for Children and Adolescents, Children's Hospital, Goethe University, Frankfurt, Germany.
Goethe University Frankfurt · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Ataxia telangiectasia is a genetic instability syndrome characterized by neurodegeneration, immunodeficiency, severe bronchial complications, hypersensitivity to radiotherapy and an elevated risk of malignancies. Repopulation with ATM-competent bone marrow-derived cells (BMDCs) significantly prolonged the lifespan and improved the phenotype of Atm-deficient mice. The aim of the present study was to promote BMDC engraftment after bone marrow transplantation using low-dose irradiation (IR) as a co-conditioning strategy. Atm-deficient mice were transplanted with green fluorescent protein-expressing, ATM-positive BMDCs using a clinically relevant non-myeloablative host-conditioning regimen together with TBI (0.2-2.0 Gy). IR significantly improved the engraftment of BMDCs into the bone marrow, blood, spleen and lung in a dose-dependent manner, but not into the cerebellum. However, with increasing doses, IR lethality increased even after low-dose IR. Analysis of the bronchoalveolar lavage fluid and lung histochemistry revealed a significant enhancement in the number of inflammatory cells and oxidative damage. A delay in the resolution of γ-H2AX-expression points to an insufficient double-strand break repair capacity following IR with 0.5 Gy in Atm-deficient splenocytes. Our results demonstrate that even low-dose IR results in ATM activation. In the absence of ATM, low-dose IR leads to increased inflammation, oxidative stress and lethality in the Atm-deficient mouse model.

Indexed as

Bone Marrow TransplantationTransplantation ConditioningWhole-Body IrradiationAllograftsAnimalsAtaxia Telangiectasia Mutated ProteinsMiceMice, Mutant StrainsAtaxia Telangiectasia Mutated ProteinsAtm protein, mouse

Identifiers

PMID26752140
OpenAlexW2239541667

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.