ArticleNature communications2016
A simple and versatile design concept for fluorophore derivatives with intramolecular photostabilization.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 44 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
44 citing papers in PubMed.
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- Allosteric Regulation of Photophysics and Binding in Oxazine-macrocycle Complexes at Single-molecule Resolution.Angewandte Chemie (International ed. in English) · 2026Article
- Covalent ferrocene conjugation as an intramolecular strategy for photostability in fluorescein.Scientific reports · 2026Article
- Advancing Single-Molecule Biophysics: Next-Generation Organic Fluorophores with Tailored Labeling Strategies.Chemical & biomedical imaging · 2025Review
- Article
- Unraveling the structure-property relationships in fluorescent phenylhydrazones: the role of substituents and molecular interactions.RSC advances · 2025Article
- Minimally Invasive DNA-Mediated Photostabilization for Extended Single-Molecule and Super-resolution Imaging.bioRxiv : the preprint server for biology · 2025Article
- Gentle Rhodamines for Live-Cell Fluorescence Microscopy.ACS central science · 2024Article
- Scanning single molecule localization microscopy (scanSMLM) for super-resolution volume imaging.Communications biology · 2023Article
- Amphiphilic Fluorinated Unimer Micelles as Nanocarriers of Fluorescent Probes for Bioimaging.ACS applied nano materials · 2023Article
- Rationalizing the environment-dependent photophysical behavior of a DNA luminescent probe by classical and non-adiabatic molecular dynamics simulations.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- AT-specific DNA visualization revisits the directionality of bacteriophage λ DNA ejection.Nucleic acids research · 2023Article
- Photochemical Mechanisms of Fluorophores Employed in Single-Molecule Localization Microscopy.Angewandte Chemie (International ed. in English) · 2023Review
- Leveraging Baird aromaticity for advancement of bioimaging applications.Journal of physical organic chemistry · 2023Article
- Improved Dye Survival in Expansion Microscopy through Stabilizer-Conjugated Linkers.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- Cross-validation of distance measurements in proteins by PELDOR/DEER and single-molecule FRET.Nature communications · 2022Article
- Linker Molecules Convert Commercial Fluorophores into Tailored Functional Probes during Biolabelling.Angewandte Chemie (International ed. in English) · 2022Article
- Targetable Conformationally Restricted Cyanines Enable Photon-Count-Limited Applications*.Angewandte Chemie (International ed. in English) · 2021Article
- Characterization of Fluorescent Proteins with Intramolecular Photostabilization*.Chembiochem : a European journal of chemical biology · 2021Article
- Cell-Permeable Nanobodies Allow Dual-Color Super-Resolution Microscopy in Untransfected Living Cells.Angewandte Chemie (International ed. in English) · 2021Article
Corrections and comments
- Erratum issued
- Erratum issued
- Erratum issued
Authors and funding
12 authors.
Funding
Abstract
Intramolecular photostabilization via triple-state quenching was recently revived as a tool to impart synthetic organic fluorophores with 'self-healing' properties. To date, utilization of such fluorophore derivatives is rare due to their elaborate multi-step synthesis. Here we present a general strategy to covalently link a synthetic organic fluorophore simultaneously to a photostabilizer and biomolecular target via unnatural amino acids. The modular approach uses commercially available starting materials and simple chemical transformations. The resulting photostabilizer-dye conjugates are based on rhodamines, carbopyronines and cyanines with excellent photophysical properties, that is, high photostability and minimal signal fluctuations. Their versatile use is demonstrated by single-step labelling of DNA, antibodies and proteins, as well as applications in single-molecule and super-resolution fluorescence microscopy. We are convinced that the presented scaffolding strategy and the improved characteristics of the conjugates in applications will trigger the broader use of intramolecular photostabilization and help to emerge this approach as a new gold standard.
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