ArticleSoft matter2016
Characterization of the crosslinking kinetics of multi-arm poly(ethylene glycol) hydrogels formed via Michael-type addition.
Article in Soft matter, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Engineering Multilayer Poly(ethylene glycol) Hydrogels via Off-Stoichiometry Thiol-Ene Chemistry.ACS applied polymer materials · 2026Article
- Small Molecule Cancer Drugs as Dual-Responsive, Dynamic-Covalent Cross-Linkers for PEG Hydrogels.ACS omega · 2026Article
- Degradable multi-arm PEG hydrogels with tunable stiffness and diffusivity.Biomaterials science · 2026Article
- Replacing the vitreous body with hydrogels: Rationale and strategies.Progress in retinal and eye research · 2025Review
- Designing Dynamic Hydrogels: The Interplay of Cross-Linker Length, Valency, and Reaction Kinetics in Hydrazone-Based Networks.Chemistry of materials : a publication of the American Chemical Society · 2025Article
- Review
- Architectural engineering of Cyborg Bacteria with intracellular hydrogel.Materials today. Bio · 2024Article
- Macroporous PEG-Alginate Hybrid Double-Network Cryogels with Tunable Degradation Rates Prepared via Radical-Free Cross-Linking for Cartilage Tissue Engineering.ACS applied bio materials · 2024Article
- Engineered Synthetic Matrices for Human Intestinal Organoid Culture and Therapeutic Delivery.Advanced materials (Deerfield Beach, Fla.) · 2024Review
- The Effect of PEGylation on Drugs' Pharmacokinetic Parameters; from Absorption to Excretion.Current drug delivery · 2024Review
- New Solutions for Old Problems: How Reproductive Tissue Engineering Has Been Revolutionizing Reproductive Medicine.Annals of biomedical engineering · 2023Review
- A combination of matrix stiffness and degradability dictate microvascular network assembly and remodeling in cell-laden poly(ethylene glycol) hydrogels.Biomaterials · 2023Article
- Protease-degradable hydrogels with multifunctional biomimetic peptides for bone tissue engineering.Frontiers in bioengineering and biotechnology · 2023Article
- Extracellular Optogenetics at the Interface of Synthetic Biology and Materials Science.Frontiers in bioengineering and biotechnology · 2022Review
- A poly(ethylene glycol) three-dimensional bone marrow hydrogel.Biomaterials · 2022Article
- Regulating the Homogeneity of Thiol-Maleimide Michael-Type Addition-Based Hydrogels Using Amino Biomolecules.Gels (Basel, Switzerland) · 2021Article
- Engineering hydrogels with homogeneous mechanical properties for controlling stem cell lineage specification.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Solute Transport Dependence on 3D Geometry of Hydrogel Networks.Macromolecular chemistry and physics · 2021Article
- Natural Presentation of Glycosaminoglycans in Synthetic Matrices for 3D Angiogenesis Models.Frontiers in cell and developmental biology · 2021Article
- 3D Silk Fiber Construct Embedded Dual-Layer PEG Hydrogel for Articular Cartilage Repair -Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Tunable properties of multi-arm poly(ethylene glycol) (PEG) hydrogel, crosslinked by Michael-type addition, support diverse applications in tissue engineering. Bioactive modification of PEG is achieved by incorporating integrin binding sequences, like RGD, and crosslinking with tri-functional protease sensitive crosslinking peptide (GCYKNRGCYKNRCG), which compete for the same reactive groups in PEG. This competition leads to a narrow range of conditions that support sufficient crosslinking density to provide structural control. Kinetics of hydrogel formation plays an important role in defining the conditions to form hydrogels with desired mechanical and biological properties, which have not been fully characterized. In this study, we explored how increasing PEG functionality from 4 to 8-arms and the concentration of biological moieties, ranging from 0.5 mM to 3.75 mM, affected the kinetics of hydrogel formation, storage modulus, and swelling after the hydrogels were allowed to form for 15 or 60 minutes. Next, human bone marrow stromal cells were encapsulated and cultured in these modified hydrogels to investigate the combined effect of mechano-biological properties on phenotypes of encapsulated cells. While the molar concentration of the reactive functional groups (-vinyl sulfone) was identical in the conditions comparing 4 and 8-arm PEG, the 8-arm PEG formed faster, allowed a greater degree of modification, and was superior in three-dimensional culture. The degrees of swelling and storage modulus of 8-arm PEG were less affected by the modification compared to 4-arm PEG. These findings suggest that 8-arm PEG allows a more precise control of mechanical properties that could lead to a larger spectrum of tissue engineering applications.
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