ArticleScientific reports2016
A quantitative investigation of linker histone interactions with nucleosomes and chromatin.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed.
- Linker histone H1.5 contributes to centromere integrity.Nucleic acids research · 2026Article
- Energy Landscapes and Structural Plasticity of Intrinsically Disordered Histones.Journal of chemical information and modeling · 2025Article
- HMGB1 deforms nucleosomal DNA to generate a dynamic chromatin environment counteracting the effects of linker histone.Science advances · 2025Article
- Altering metabolism programs cell identity via NADThe EMBO journal · 2025Article
- Characterization of Medusavirus encoded histones reveals nucleosome-like structures and a unique linker histone.Nature communications · 2024Article
- A DNA condensation code for linker histones.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Regulation of chromatin architecture by protein binding: insights from molecular modeling.Biophysical reviews · 2024Review
- Real-Time Multistep Asymmetrical Disassembly of Nucleosomes and Chromatosomes Visualized by High-Speed Atomic Force Microscopy.ACS central science · 2024Article
- Interactome of intact chromatosome variants with site-specifically ubiquitylated and acetylated linker histone H1.2.Nucleic acids research · 2024Article
- Cell cycle-dependent gene networks for cell proliferation activated by nuclear CK2α complexes.Life science alliance · 2024Article
- Variability of Inverted Repeats in All Available Genomes of Bacteria.Microbiology spectrum · 2023Article
- Development of Convergent Hybrid Phase Ligation for Efficient and Convenient Total Synthesis of Proteins.Peptide science (Hoboken, N.J.) · 2023Article
- Simulating the chromatin-mediated phase separation of model proteins with multiple domains.Biophysical journal · 2022Article
- Single-stranded nucleic acid binding and coacervation by linker histone H1.Nature structural & molecular biology · 2022Article
- H1.0 C Terminal Domain Is Integral for Altering Transcription Factor Binding within Nucleosomes.Biochemistry · 2022Article
- A Protein Semisynthesis-Based Strategy to Investigate the Functional Impact of Linker Histone Serine ADP-Ribosylation.ACS chemical biology · 2022Article
- Histone variants: The unsung guardians of the genome.DNA repair · 2022Review
- Release of linker histone from the nucleosome driven by polyelectrolyte competition with a disordered protein.Nature chemistry · 2022Article
- SUMOylation of linker histone H1 drives chromatin condensation and restriction of embryonic cell fate identity.Molecular cell · 2022Article
- Genome-wide characterization and evolutionary analysis of linker histones in castor bean (Frontiers in plant science · 2022Article
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3 authors.
Funding
Abstract
Linker histones such as H1 are abundant basic proteins that bind tightly to nucleosomes, thereby acting as key organizers of chromatin structure. The molecular details of linker histone interactions with the nucleosome, and in particular the contributions of linker DNA and of the basic C-terminal tail of H1, are controversial. Here we combine rigorous solution-state binding assays with native gel electrophoresis and Atomic Force Microscopy, to quantify the interaction of H1 with chromatin. We find that H1 binds nucleosomes and nucleosomal arrays with very tight affinity by recognizing a specific DNA geometry minimally consisting of a solitary nucleosome with a single ~18 base pair DNA linker arm. The association of H1 alters the conformation of trinucleosomes so that only one H1 can bind to the two available linker DNA regions. Neither incorporation of the histone variant H2A.Z, nor the presence of neighboring nucleosomes affects H1 affinity. Our data provide a comprehensive thermodynamic framework for this ubiquitous chromatin architectural protein.
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