ArticleScientific reports2016
Bright and photostable push-pull pyrene dye visualizes lipid order variation between plasma and intracellular membranes.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
55 citing papers in PubMed.
- Electric field-dependent excited-state twisting in donor-acceptor thiazolothiazole dyes establishes a new class of cell membrane voltage sensors.Chemical science · 2026Article
- Bright Solvatochromic Fluorophores With Dual Emission Enabled by a Donor-Engineering Strategy.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- Fluorescence Analysis of Local Microenvironments in Polymer Films Using Solvatochromic Dyes.Sensors (Basel, Switzerland) · 2026Article
- Lipid Metabolism and Trafficking in the Golgi Apparatus.Sub-cellular biochemistry · 2026Review
- Trifluoroacetyl-Substituted D-π-A Fluorophores: Design Strategies for Strong Solvatochromism and Giga Stokes Shifts.Chemistry, an Asian journal · 2025Article
- Membrane order regulates clathrin-coated pit dynamics but not initiation.Molecular biology of the cell · 2025Article
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- Synaptophysin accelerates synaptic vesicle fusion by expanding the membrane upon neurotransmitter loading.Science advances · 2025Article
- D-π-A Fluorophores with Strong Solvatochromism for Single-Molecule Ratiometric Thermometers.Journal of the American Chemical Society · 2025Article
- An exchangeable SIM probe for monitoring organellar dynamics of necrosis cells and intracellular water heterogeneity in kidney repair.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Exploring Imaging Applications of a Red-Emitting π-Acceptor (π-A) Pyrene-Benzothiazolium Dye.Biosensors · 2024Article
- Multifocal lipid membrane characterization by combination of DAS-deconvolution and anisotropy.Biophysical journal · 2024Article
- Membrane-mediated modulation of mitochondrial physiology by terahertz waves.Biomedical optics express · 2024Article
- Fluorescent Solvatochromic Probes for Long-Term Imaging of Lipid Order in Living Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Serinc5 Restricts HIV Membrane Fusion by Altering Lipid Order and Heterogeneity in the Viral Membrane.ACS infectious diseases · 2023Article
- Decomposition analysis on the excitation behaviors of thiazolothiazole (TTz)-based dyesRSC advances · 2022Article
- Flexible Alkylene Bridges as a Tool To Engineer Crystal Distyrylbenzene Structures Enabling Highly Fluorescent Monomeric Emission.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Article
- The Unexpected Selectivity Switching from Mitochondria to Lysosome in a D-π-A Cyanine Dye.Biosensors · 2022Article
- Dynamic covalent chemistry in live cells for organelle targeting and enhanced photodynamic action.Chemical science · 2022Article
- Erythrocyte Membrane Nanomechanical Rigidity Is Decreased in Obese Patients.International journal of molecular sciences · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
Imaging lipid organization in cell membranes requires advanced fluorescent probes. Here, we show that a recently synthesized push-pull pyrene (PA), similarly to popular probe Laurdan, changes the emission maximum as a function of lipid order, but outperforms it by spectroscopic properties. In addition to red-shifted absorption compatible with common 405 nm diode laser, PA shows higher brightness and much higher photostability than Laurdan in apolar membrane environments. Moreover, PA is compatible with two-photon excitation at wavelengths >800 nm, which was successfully used for ratiometric imaging of coexisting liquid ordered and disordered phases in giant unilamellar vesicles. Fluorescence confocal microscopy in Hela cells revealed that PA efficiently stains the plasma membrane and the intracellular membranes at >20-fold lower concentrations, as compared to Laurdan. Finally, ratiometric imaging using PA reveals variation of lipid order within different cellular compartments: plasma membranes are close to liquid ordered phase of model membranes composed of sphingomyelin and cholesterol, while intracellular membranes are much less ordered, matching well membranes composed of unsaturated phospholipids without cholesterol. These differences in the lipid order were confirmed by fluorescence lifetime imaging (FLIM) at the blue edge of PA emission band. PA probe constitutes thus a new powerful tool for biomembrane research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.