Evidence map›Paper›PMID 26750120›Full record

ArticleCurrent microbiology2016

Cloning, Expression, Purification, Regulation, and Subcellular Localization of a Mini-protein from Campylobacter jejuni.

Soumeya Aliouane, Jean-Marie Pagès, Jean-Michel Bolla

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Soumeya AliouaneUMR-MD1 Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille Cedex 05, France.
Jean-Marie PagèsUMR-MD1 Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille Cedex 05, France.
Jean-Michel BollaUMR-MD1 Faculté de Médecine, 27 Bd Jean Moulin, 13385, Marseille Cedex 05, France. jean-michel.bolla@univ-amu.fr.
Université Jean Moulin Lyon III · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Cj1169c-encoded putative protein of Campylobacter was expressed and purified from E. coli after sequence optimization. The purified protein allowed the production of a specific rabbit antiserum that was used to study the protein expression in vitro and its subcellular localization in the bacterial cell and putative interactions with other proteins. This protein is produced in Campylobacter and it clearly localizes into the periplasmic space. The level of protein production depends on factors, including pH, temperature, osmolarity, and growth phase suggesting a role in the Campylobacter environmental adaptation. The cysteine residues present in the sequence are probably involved in disulfide bridges, which may promote covalent interactions with other proteins of the Campylobacter envelope.

Indexed as

Gene Expression Regulation, BacterialAmino Acid MotifsAnimalsBacterial ProteinsCampylobacter jejuniCloning, MolecularEscherichia coliHydrogen-Ion ConcentrationPeriplasmProtein TransportRabbitsBacterial Proteins

Identifiers

PMID26750120
OpenAlexW2239924261

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.