ArticleThe Journal of biological chemistry2016
Coordinative Modulation of Chlorothricin Biosynthesis by Binding of the Glycosylated Intermediates and End Product to a Responsive Regulator ChlF1.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 33 citations in OpenAlex.
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- Study on the regulatory mechanism of NsdAMicrobial cell factories · 2025Article
- Identification of a novel butenolide signal system to regulate high production of tylosin in Streptomyces fradiae.Applied microbiology and biotechnology · 2025Article
- The LysR family transcriptional regulator ORF-L16 regulates spinosad biosynthesis inSynthetic and systems biotechnology · 2024Article
- NeoI represents a group of transcriptional repressors regulating the biosynthesis of multiple aminoglycosides.Science China. Life sciences · 2024Article
- Cinnamoyl lipids as novel signaling molecules modulate the physiological metabolism of cross-phylum microorganisms.Communications biology · 2024Article
- Article
- Multi-omics Data Reveal the Effect of Sodium Butyrate on Gene Expression and Protein Modification in Streptomyces.Genomics, proteomics & bioinformatics · 2023Article
- Phylogenetic classification of natural product biosynthetic gene clusters based on regulatory mechanisms.Frontiers in microbiology · 2023Article
- Identification of a Novel Pleiotropic Transcriptional Regulator Involved in Sporulation and Secondary Metabolism Production inInternational journal of molecular sciences · 2022Article
- A Glossary for Chemical Approaches towards Unlocking the Trove of Metabolic Treasures inMolecules (Basel, Switzerland) · 2021Review
- Molecular mechanism of mureidomycin biosynthesis activated by introduction of an exogenous regulatory gene ssaA into Streptomyces roseosporus.Science China. Life sciences · 2021Article
- Reconstitution of a mini-gene cluster combined with ribosome engineering led to effective enhancement of salinomycin production in Streptomyces albus.Microbial biotechnology · 2021Article
- Activation of Cryptic Antibiotic Biosynthetic Gene Clusters Guided by RNA-seq Data from BothAntibiotics (Basel, Switzerland) · 2021Article
- Identification and characterization of deschloro-chlorothricin obtained from a large natural product library targeting aurora A kinase in multiple myeloma.Investigational new drugs · 2021Article
- Co-expression of a SARP Family Activator ChlF2 and a Type II Thioesterase ChlK Led to High Production of Chlorothricin inFrontiers in bioengineering and biotechnology · 2020Article
- AcrR and Rex Control Mannitol and Sorbitol Utilization through Their Cross-Regulation of Aldehyde-Alcohol Dehydrogenase (AdhE) in Lactobacillus plantarum.Applied and environmental microbiology · 2019Article
- Regulation of Antibiotic Production by Signaling Molecules inFrontiers in microbiology · 2019Review
- Identification of a butenolide signaling system that regulates nikkomycin biosynthesis inThe Journal of biological chemistry · 2018Article
- Regulation of antibiotic biosynthesis in actinomycetes: Perspectives and challenges.Synthetic and systems biotechnology · 2018Review
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chlorothricin, isolated from Streptomyces antibioticus, is a parent member of spirotetronate family of antibiotics that have long been appreciated for their remarkable biological activities. ChlF1 plays bifunctional roles in chlorothricin biosynthesis by binding to its target genes (chlJ, chlF1, chlG, and chlK). The dissociation constants of ChlF1 to these genes are ∼ 102-140 nm. A consensus sequence, 5'-GTAANNATTTAC-3', was found in these binding sites. ChlF1 represses the transcription of chlF1, chlG, and chlK but activates chlJ, which encodes a key enzyme acyl-CoA carboxyl transferase involved in the chlorothricin biosynthesis. We demonstrate that the end product chlorothricin and likewise its biosynthetic intermediates (demethylsalicycloyl chlorothricin and deschloro-chlorothricin) can act as signaling molecules to modulate the binding of ChlF1 to its target genes. Intriguingly, a correlation between the antibacterial activity and binding ability of signaling molecules to the regulator ChlF1 is clearly observed. These features of the signaling molecules are associated with the glycosylation of spirotetronate macrolide aglycone. The findings provide new insights into the TetR family regulators responding to special structure of signaling molecules, and we reveal the regulatory mini-network mediated by ChlF1 in chlorothricin biosynthesis for the first time.
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