ArticleJournal of industrial microbiology & biotechnology2016
Decreased fluidity of cell membranes causes a metal ion deficiency in recombinant Saccharomyces cerevisiae producing carotenoids.
Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 39 citations in OpenAlex.
- Multiomics reveals the molecular mechanism of unsaturated fatty acid-induced terpenoid biosynthesis in Sanghuangporus lonicericola.NPJ science of food · 2025Article
- A transcriptome-guided genetic engineering strategy to balance cell growth with astaxanthin production inFrontiers in microbiology · 2025Article
- Transcriptomic and Metabolomic Profiling Uncovers Response Mechanisms of Alicyclobacillus acidoterrestris DSM 3922Microbiology spectrum · 2023Article
- Metabolic Engineering of Model Microorganisms for the Production of Xanthophyll.Microorganisms · 2023Review
- Compartmentalization engineering of yeasts to overcome precursor limitations and cytotoxicity in terpenoid production.Frontiers in bioengineering and biotechnology · 2023Review
- Optimization of microbial cell factories for astaxanthin production: Biosynthesis and regulations, engineering strategies and fermentation optimization strategies.Synthetic and systems biotechnology · 2022Review
- Compartmentalization and transporter engineering strategies for terpenoid synthesis.Microbial cell factories · 2022Review
- Biotechnological advances for improving natural pigment production: a state-of-the-art review.Bioresources and bioprocessing · 2022Review
- Dual regulation of lipid droplet-triacylglycerol metabolism and ERG9 expression for improved β-carotene production in Saccharomyces cerevisiae.Microbial cell factories · 2022Article
- Efficient multiplexed gene regulation in Saccharomyces cerevisiae using dCas12a.Nucleic acids research · 2021Article
- The Plasma Membrane at the Cornerstone Between Flexibility and Adaptability: Implications forFrontiers in microbiology · 2021Review
- Analysis ofMetabolic engineering communications · 2020Article
- Application of Stable Isotope Tracing to Elucidate Metabolic Dynamics During Yarrowia lipolytica α-Ionone Fermentation.iScience · 2020Article
- Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene inBiotechnology for biofuels · 2020Article
- Primary and Secondary Metabolic Effects of a Key Gene Deletion (ΔApplied and environmental microbiology · 2019Article
- Lessons in Membrane Engineering for Octanoic Acid Production from Environmental Escherichia coli Isolates.Applied and environmental microbiology · 2018Article
- Crossing boundaries: the importance of cellular membranes in industrial biotechnology.Journal of industrial microbiology & biotechnology · 2017Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome-wide transcriptional responses of S. cerevisiae to heterologous carotenoid biosynthesis were investigated using DNA microarray analysis. The results show that the genes involved in metal ion transport were specifically up-regulated in the recombinant strain, and metal ions, including Cu(2+), Fe(2+), Mn(2+), and Mg(2+), were deficient in the recombinant strain compared to the ion content of the parent strain. The decrease in metal ions was ascribed to a decrease in cell membrane (CM) fluidity caused by lower levels of unsaturated fatty acids and ergosterol. This was confirmed by the observation that metal ion levels were restored when CM fluidity was increased by supplying linoleic acid. In addition, a 24.3 % increase in the β-carotene concentration was observed. Collectively, our results suggest that heterologous production of carotenoids in S. cerevisiae can induce cellular stress by rigidifying the CM, which can lead to a deficiency in metal ions. Due to the importance of CM fluidity in cellular physiology, maintaining normal CM fluidity might be a potential approach to improving carotenoid production in genetically engineered S. cerevisiae.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.