ArticleJournal of biomedical materials research. Part A2016
Tuning microenvironment modulus and biochemical composition promotes human mesenchymal stem cell tenogenic differentiation.
Article in Journal of biomedical materials research. Part A, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed.
- High-throughput bioprinted 3D cultures for probing host-pathogen interactions in bioinspired microenvironments.RSC applied polymers · 2026Article
- Triple Helical-Peptides: Collagen-Mimicking Tools for Tissue Engineering.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Transcriptome-Optimized Hydrogel Design of a Stem Cell Niche for Enhanced Tendon Regeneration.Advanced materials (Deerfield Beach, Fla.) · 2025Article
- Hierarchy Reproduction: Multiphasic Strategies for Tendon/Ligament-Bone Junction Repair.Biomaterials research · 2025Review
- Controlled Stiffness of Direct-Write, Near-Field Electrospun Gelatin Fibers Generates Differences in Tenocyte Morphology and Gene Expression.Journal of biomechanical engineering · 2024Article
- Soft Polyethylene Glycol Hydrogels Support Human PSC Pluripotency and Morphogenesis.ACS biomaterials science & engineering · 2024Article
- Tissue-engineered mesenchymal stem cell constructs alleviate tendinopathy by suppressing vascularization.Bioactive materials · 2024Article
- Injectable liposome-containing click hydrogel microparticles for release of macromolecular cargos.Soft matter · 2024Article
- Structurally decoupled stiffness and solute transport in multi-arm poly(ethylene glycol) hydrogels.Biomaterials · 2023Article
- Systematic d-Amino Acid Substitutions to Control Peptide and Hydrogel Degradation in Cellular Microenvironments.ACS macro letters · 2023Article
- The Stiffness-Sensitive Transcriptome of Human Tendon Stromal Cells.Advanced healthcare materials · 2023Article
- Applications of functionally-adapted hydrogels in tendon repair.Frontiers in bioengineering and biotechnology · 2023Review
- Article
- Destructive fibrotic teamwork: how both microenvironment stiffness and profibrotic interleukin 13 impair alveolar macrophage phenotype and function.Biomaterials science · 2022Article
- Biomechanically and biochemically functional scaffold for recruitment of endogenous stem cells to promote tendon regeneration.NPJ Regenerative medicine · 2022Article
- Role of Yes-associated protein (YAP) in regulation of mesenchymal stem cell tenogenic differentiation.Journal of molecular histology · 2022Article
- Multiscale Invasion Assay for Probing Macrophage Response to Gram-Negative Bacteria.Frontiers in chemistry · 2022Article
- Success Criteria and Preclinical Testing of Multifunctional Hydrogels for Tendon Regeneration.Tissue engineering. Part C, Methods · 2020Article
- Designing Microgels for Cell Culture and Controlled Assembly of Tissue Microenvironments.Advanced functional materials · 2020Article
- Enhanced tenogenic differentiation and tendon-like tissue formation by Scleraxis overexpression in human amniotic mesenchymal stem cells.Journal of molecular histology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Mesenchymal stem cells (MSCs) are promising for the regeneration of tendon and ligament tissues. Toward realizing this potential, microenvironment conditions are needed for promoting robust lineage-specific differentiation into tenocytes/ligament fibroblasts. Here, we utilized a statistical design of experiments approach to examine combinations of matrix modulus, composition, and soluble factors in human MSC tenogenic/ligamentogenic differentiation. Specifically, well-defined poly(ethylene glycol)-based hydrogels were synthesized using thiol-ene chemistry providing a bioinert base for probing cell response to extracellular matrix cues. Monomer concentrations were varied to achieve a range of matrix moduli (E ∼ 10-90 kPa), and different ratios of integrin-binding peptides were incorporated (GFOGER and RGDS for collagen and fibronectin, respectively), mimicking aspects of developing tendon/ligament tissue. A face-centered central composite response surface design was utilized to understand the contributions of these cues to human MSC differentiation in the presence of soluble factors identified to promote tenogenesis/ligamentogenesis (BMP-13 and ascorbic acid). Increasing modulus and collagen mimetic peptide content increased relevant gene expression and protein production or retention (scleraxis, collagen I, tenascin-C). These findings could inform the design of materials for tendon/ligament regeneration. More broadly, the design of experiments enabled efficient data acquisition and analysis, requiring fewer replicates than if each factor had been varied one at a time. This approach can be combined with other stimuli (for example, mechanical stimulation) toward a better mechanistic understanding of differentiation down these challenging lineages.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.