ArticleInvestigative ophthalmology & visual science2015
Effect of Stratification on Surface Properties of Corneal Epithelial Cells.
Article in Investigative ophthalmology & visual science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Functional and morphological evaluation of the meibomian glands and ocular surface assessment at high altitude.Indian journal of ophthalmology · 2023Trial
- Delamination of epithelia induced by air-liquid interfaces.Molecular biology of the cell · 2025Article
- The role of epithelial membrane-associated mucin 4 in ocular surface health and corneal wound healing.Frontiers in medicine · 2025Article
- Meibomian gland lipid alterations and ocular surface sequela in Awat2 knockout murine model of meibomian gland dysfunction and evaporative dry eye disease.The ocular surface · 2024Article
- Elucidating the roles of electrolytes and hydrogen bonding in the dewetting dynamics of the tear film.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Bioinspired Lubricity from Surface Gel Layers.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- A Mucin-Deficient Ocular Surface Mimetic Platform for Interrogating Drug Effects on Biolubrication, Antiadhesion Properties, and Barrier Functionality.ACS applied materials & interfaces · 2022Article
- Human ocular mucins: The endowed guardians of sight.Advanced drug delivery reviews · 2022Review
- CRISPR/Cas9 genome editing reveals an essential role for basigin in maintaining a nonkeratinized squamous epithelium in cornea.FASEB bioAdvances · 2021Article
- Hydrogel-Supported, Engineered Model of Vocal Fold Epithelium.ACS biomaterials science & engineering · 2021Article
- Mucin-Like Glycoproteins Modulate Interfacial Properties of a Mimetic Ocular Epithelial Surface.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Overriding native cell coordination enhances external programming of collective cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Relationships between the material properties of silicone hydrogels: Desiccation, wettability and lubricity.Journal of biomaterials applications · 2021Article
- Differential effects of Hsp90 inhibition on corneal cells in vitro and in vivo.Experimental eye research · 2021Article
- Membrane-associated mucins of the ocular surface: New genes, new protein functions and new biological roles in human and mouse.Progress in retinal and eye research · 2020Review
- Cellular therapy of corneal epithelial defect by adipose mesenchymal stem cell-derived epithelial progenitors.Stem cell research & therapy · 2020Article
- Contribution of Mucins towards the Physical Properties of the Tear Film: A Modern Update.International journal of molecular sciences · 2019Review
- Article
- Mathematical modelling of glob-driven tear film breakup.Mathematical medicine and biology : a journal of the IMA · 2019Article
- Tear-film-oriented diagnosis for dry eye.Japanese journal of ophthalmology · 2019Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
purposeThe purpose of this study was to determine the influence of mucin expression in an immortalized human corneal epithelial cell line (hTCEpi) on the surface properties of cells, such as wettability, contact angle, and surface heterogeneity.
methodshTCEpi cells were cultured to confluence in serum-free medium. The medium was then replaced by stratification medium to induce mucin biosynthesis. The mucin expression profile was analyzed using quantitative PCR and Western blotting. Contact angles were measured using a two-immiscible liquid method, and contact angle hysteresis was evaluated by tilting the apparatus and recording advancing and receding contact angles. The spatial distribution of mucins was evaluated with fluorescently labeled lectin.
resultshTCEpi cells expressed the three main ocular mucins (MUC1, MUC4, and MUC16) with a maximum between days 1 and 3 of the stratification process. Upon stratification, cells caused a very significant increase in contact angle hysteresis, suggesting the development of spatially discrete and heterogeneously distributed surface features, defined by topography and/or chemical functionality. Although atomic force microscopy measurements showed no formation of appreciable topographic features on the surface of the cells, we observed a significant increase in surface chemical heterogeneity.
conclusionsThe surface chemical heterogeneity of the corneal epithelium may influence the dynamic behavior of tear film by "pinning" the contact line between the cellular surface and aqueous tear film. Engineering the surface properties of corneal epithelium could potentially lead to novel treatments in dry eye disease.
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