ArticleMolecular & cellular proteomics : MCP2016
Comparative Study of Early Cold-Regulated Proteins by Two-Dimensional Difference Gel Electrophoresis Reveals a Key Role for Phospholipase Dα1 in Mediating Cold Acclimation Signaling Pathway in Rice.
Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 56 citations in OpenAlex.
- Phosphatidic Acid Serves as an Early Signaling Molecule Involved in Membrane Lipid Metabolism to Alleviate Postharvest Chilling Injury in Peach Fruit.Foods (Basel, Switzerland) · 2026Article
- Proteomic profiling reveals important regulators of photosynthate accumulation in wheat leaves during grain development.BMC plant biology · 2025Article
- Cold stress in rice (Frontiers in plant science · 2025Review
- Identification of Candidate Genes for Cold Tolerance at Seedling Stage by GWAS in Rice (Biology · 2024Article
- Knockout of the Chlorophyll a Oxygenase GeneGenes · 2024Article
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- Comparative transcriptomics and co-expression networks reveal cultivar-specific molecular signatures associated with reproductive-stage cold stress in rice.Plant cell reports · 2023Article
- Cell Membrane Features as Potential Breeding Targets to Improve Cold Germination Ability of Seeds.Plants (Basel, Switzerland) · 2022Review
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- ITRAQ-based quantitative proteomic analysis ofBreeding science · 2022Article
- Data-Independent Acquisition-Based Proteome and Phosphoproteome Profiling Reveals Early Protein Phosphorylation and Dephosphorylation Events inInternational journal of molecular sciences · 2021Article
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- Convergence and Divergence: Signal Perception and Transduction Mechanisms of Cold Stress inPlants (Basel, Switzerland) · 2021Review
- Evolutionary analysis and functional characterization of SiBRI1 as a Brassinosteroid receptor gene in foxtail millet.BMC plant biology · 2021Article
- Heterologous expression of heat stress-responsive AtPLC9 confers heat tolerance in transgenic rice.BMC plant biology · 2020Article
- Decreased R:FR Ratio in Incident White Light Affects the Composition of Barley Leaf Lipidome and Freezing Tolerance in a Temperature-Dependent Manner.International journal of molecular sciences · 2020Article
- Response of photosynthetic capacity and antioxidative system of chloroplast in two wucai (Physiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Recent Advances in the Cellular and Developmental Biology of Phospholipases in Plants.Frontiers in plant science · 2019Review
- Proteomic Analysis of ArabidopsisFrontiers in plant science · 2019Article
- Shot-Gun Proteomic Analysis on Roots of ArabidopsisInternational journal of molecular sciences · 2018Article
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Authors and funding
10 authors at 5 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
To understand the early signaling steps that regulate cold responses in rice, two-dimensional difference gel electrophoresis (2-D DIGE)(1)was used to study early cold-regulated proteins in rice seedlings. Using mass spectrometry, 32 spots, which represent 26 unique proteins that showed an altered expression level within 5 min of cold treatment were identified. Among these proteins, Western blot analyses confirmed that the cellular phospholipase D α1 (OsPLDα1) protein level was increased as early as 1 min after cold treatment. Genetic studies showed that reducing the expression ofOsPLDα1makes rice plants more sensitive to chilling stress as well as cold acclimation increased freezing tolerance. Correspondingly, cold-regulated proteomic changes and the expression of the cold-responsive C repeat/dehydration-responsive element binding 1 (OsDREB1) family of transcription factors were inhibited in thepldα1mutant. We also found that the expression ofOsPLDα1is directly regulated by OsDREB1A. This transcriptional regulation ofOsPLDα1could provide positive feedback regulation of the cold signal transduction pathway in rice. OsPLDα1 hydrolyzes phosphatidylcholine to produce the signal molecule phosphatidic acid (PA). By lipid-overlay assay, we demonstrated that the rice cold signaling proteins, MAP kinase 6 (OsMPK6) and OsSIZ1, bind directly to PA. Taken together, our results suggest that OsPLDα1 plays a key role in transducing cold signaling in rice by producing PA and regulatingOsDREB1s' expression by OsMPK6, OsSIZ1, and possibly other PA-binding proteins.
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