Evidence map›Paper›PMID 26747395›Full record

ArticleMolecular and biochemical parasitology2015

Expression of nicotinic acetylcholine receptor subunits from parasitic nematodes in Caenorhabditis elegans.

Megan A Sloan, Barbara J Reaves, Mary J Maclean, Bob E Storey, Adrian J Wolstenholme

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and biochemical parasitology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Mutations in the cell-binding motif ofmicroPublication biology · 2021
    Article
  6. Caenorhabditis elegans in anthelmintic research - Old model, new perspectives.International journal for parasitology. Drugs and drug resistance · 2020
    Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Megan A SloanDepartment of Infectious Diseases and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA.
Barbara J ReavesDepartment of Infectious Diseases and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA.
Mary J MacleanDepartment of Infectious Diseases and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA.
Bob E StoreyDepartment of Infectious Diseases and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA.
Adrian J WolstenholmeDepartment of Infectious Diseases and Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, GA 30602, USA. Electronic address: adrianw@uga.edu.

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Aric L Daul, Ann E. Rougvie · 2012 to 2026
$7.5M
Can drug targets from parasitic nematodes be usefully expressed in C. elegans?R21AI092022 · NIAID · UNIVERSITY OF GEORGIA · PI WOLSTENHOLME, ADRIAN J · 2012 to 2013
$420k
NIAID NIH HHS R21 AI092022NIH HHS P40 OD010440NIH HHS P40P40OD010440
6 · The paper itself

Abstract

The levamisole-sensitive nicotinic acetylcholine receptor present at nematode neuromuscular junctions is composed of multiple different subunits, with the exact composition varying between species. We tested the ability of two well-conserved nicotinic receptor subunits, UNC-38 and UNC-29, from Haemonchus contortus and Ascaris suum to rescue the levamisole-resistance and locomotion defects of Caenorhabditis elegans strains with null deletion mutations in the unc-38 and unc-29 genes. The parasite cDNAs were cloned downstream of the relevant C. elegans promoters and introduced into the mutant strains via biolistic transformation. The UNC-38 subunit of H. contortus was able to completely rescue both the locomotion defects and levamisole resistance of the null deletion mutant VC2937 (ok2896), but no C. elegans expressing the A. suum UNC-38 could be detected. The H. contortus UNC-29.1 subunit partially rescued the levamisole resistance of a C. elegans null mutation in unc-29 VC1944 (ok2450), but did cause increased motility in a thrashing assay. In contrast, only a single line of worms containing the A. suum UNC-29 subunit showed a partial rescue of levamisole resistance, with no effect on thrashing.

Indexed as

AnimalsAntinematodal AgentsAscaris suumBiolisticsCaenorhabditis elegansCaenorhabditis elegans ProteinsCarrier ProteinsCloning, MolecularDNA, ComplementaryDNA, HelminthDrug ResistanceHaemonchusLevamisoleLocomotionProtein SubunitsReceptors, NicotinicAntinematodal AgentsCaenorhabditis elegans ProteinsCarrier ProteinsDNA, ComplementaryDNA, HelminthLevamisoleProtein SubunitsReceptors, NicotinicUNC-29 protein, C elegansUnc-38 protein, C elegansAscaris suumCaenorhabditis elegansHaemonchus contortusLevamisoleNicotinic acetylcholine receptor

Identifiers

PMID26747395
PMCPMC4729587

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.