ArticlePlant physiology2016
Deficiency of Starch Synthase IIIa and IVb Alters Starch Granule Morphology from Polyhedral to Spherical in Rice Endosperm.
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Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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69citing papers in PubMed
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69 citing papers in PubMed, 156 citations in OpenAlex.
- In Planta Expression of Thermophilic GtGBE Mimics BEIIb and Modifies Rice Starch Structure Without Yield Penalties.Plant biotechnology journal · 2026Article
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- LIKE EARLY STARVATION is involved in the regulation of starch initiation in potato tubers (Solanum tuberosum cv. Désirée).Journal of experimental botany · 2026Article
- Genetic Determinants and Breeding Strategies for Rice Cooking and Eating Quality: A Comprehensive Review.Plant biotechnology journal · 2026Review
- High-Energy Emulsified Clove Essential Oil Nanoemulsion as a Natural Herbicidal Product: Germination Suppression and Seed Structure Alteration inPlants (Basel, Switzerland) · 2026Article
- Polyploidization-Driven Functional Innovation of AGPase Small Subunit GeneInternational journal of molecular sciences · 2026Article
- Modification of starch synthesis in food crops using CRISPR/Cas9 gene editing technology for changing climate.Stress biology · 2026Review
- Soluble starch synthase IVa determines cooked rice elongation in waxy and non-waxy rices (Frontiers in plant science · 2026Article
- Rice Genotype Related to Starch Biosynthesis: Effects on Rice Starch Structure and Enzymatic Digestibility duringJournal of applied glycoscience · 2026Article
- Integrative transcriptogenomic analyses reveal the regulatory network underlying rice eating and cooking quality and identify a role for alpha-globulin in modulating starch and sucrose metabolism.Plant communications · 2025Article
- Physiological and compensatory roles of three starch-branching enzymes in different rice organs.Plant molecular biology · 2025Article
- Creation of high-resistant starch rice through systematic editing of amylopectin biosynthetic genes in rs4.Plant biotechnology journal · 2025Article
- Development of phytochemical genomics: From decoding metabolome to functional genomics and biotechnology of plant metabolism.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2025Review
- Mutations in starch BRANCHING ENZYME 2a suppress the traits caused by the loss of ISOAMYLASE1 in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Starch Properties and Morphology of Eight Floury Endosperm Mutants in Rice.Plants (Basel, Switzerland) · 2023Article
- Customizing Starch Properties: A Review of Starch Modifications and Their Applications.Polymers · 2023Review
- A model for the reproduction of amylopectin cluster by coordinated actions of starch branching enzyme isoforms.Plant molecular biology · 2023Review
- Impacts of Inherent Components and Nitrogen Fertilizer on Eating and Cooking Quality of Rice: A Review.Foods (Basel, Switzerland) · 2023Review
- Loss of function ofProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Effect of Shearing and Heat Milling Treatment Temperature on the Crystallinity, Thermal Properties, and Molecular Structure of Rice Starch.Foods (Basel, Switzerland) · 2023Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors at 8 institutions in 2 countries.
Yoshiko ToyosawaDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Yasushi KawagoeDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Ryo MatsushimaDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Naoko CroftsDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).ORCID http://orcid.org/0000-0002-6792-2232
Masahiro OgawaDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Masako FukudaDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Toshihiro KumamaruDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Yozo OkazakiDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Miyako KusanoDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Kazuki SaitoDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Kiminori ToyookaDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Mayuko SatoDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Yongfeng AiDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).
Jay-Lin JaneDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).ORCID http://orcid.org/0000-0002-7570-2774
Yasunori NakamuraDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.).ORCID http://orcid.org/0000-0001-7660-0078
Naoko FujitaDepartment of Biological Production, Akita Prefectural University, Akita City, Akita 010-0195, Japan (Y.T., N.C., Y.N., N.F.);Division of Plant Sciences, National Institute of Agrobiological Sciences, Tsukuba 305-8602, Japan (Y.K.);Institute of Plant Science and Resources, Okayama University, Kurashiki 710-0046, Japan (R.M.);Department of General Education, Yamaguchi Prefectural University, Yamaguchi 753-8502, Japan (M.O.);Plant Genetic Resources, Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan (M.F., T.K.);RIKEN Center for Sustainable Resource Science, Tsurumi-ku, Yokohama 230-0045, Japan (Y.O., M.K., K.S., K.T., M.S.);Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba 260-8675, Japan (K.S.); andDepartment of Food Science and Human Nutrition, Iowa State University, Ames, Iowa 50011-1120 (Y.A., J.-L.J.) naokof@akita-pu.ac.jp.
Akita Prefectural University · JPRIKEN Center for Sustainable Resource Science · JPIowa State University · USKyushu University · JPChiba University · JPInstitute of Agrobiological Sciences · JPOkayama University · JPYamaguchi Prefectural University · JP
Funding
No grant is acknowledged in the PubMed record.
Abstract
Starch granule morphology differs markedly among plant species. However, the mechanisms controlling starch granule morphology have not been elucidated. Rice (Oryza sativa) endosperm produces characteristic compound-type granules containing dozens of polyhedral starch granules within an amyloplast. Some other cereal species produce simple-type granules, in which only one starch granule is present per amyloplast. A double mutant rice deficient in the starch synthase (SS) genes SSIIIa and SSIVb (ss3a ss4b) produced spherical starch granules, whereas the parental single mutants produced polyhedral starch granules similar to the wild type. The ss3a ss4b amyloplasts contained compound-type starch granules during early developmental stages, and spherical granules were separated from each other during subsequent amyloplast development and seed dehydration. Analysis of glucan chain length distribution identified overlapping roles for SSIIIa and SSIVb in amylopectin chain synthesis, with a degree of polymerization of 42 or greater. Confocal fluorescence microscopy and immunoelectron microscopy of wild-type developing rice seeds revealed that the majority of SSIVb was localized between starch granules. Therefore, we propose that SSIIIa and SSIVb have crucial roles in determining starch granule morphology and in maintaining the amyloplast envelope structure. We present a model of spherical starch granule production.
Indexed as
DNA, PlantEndospermLipid MetabolismMicroscopy, Electron, ScanningMicroscopy, Electron, TransmissionMutationOryzaPhylogenyPlant ProteinsPlants, Genetically ModifiedPlastidsRecombinant ProteinsStarchStarch SynthaseDNA, PlantPlant ProteinsRecombinant ProteinsStarchStarch Synthase
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