ArticleApplied and environmental microbiology2016
Microbial Diversity in the Early In Vivo-Formed Dental Biofilm.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07307716 (Investigating the Impact of Cleaning Protocols on Oral Microbial Ecology and Early Dysbiosis Markers on Orthodontic Clear Aligner Patients), which is not on this map. Cited by 47 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Investigating the Impact of Cleaning Protocols on Oral Microbial Ecology and Early Dysbiosis Markers on Orthodontic Clear Aligner Patients: A Randomized Cross-over Clinical Study
Who cites it
47 citing papers in PubMed, 1 synthesis or guideline pooled it, 84 citations in OpenAlex.
- Use of the oral microbiota as screening test to identify children at risk for caries development. A systematic review of longitudinal studies.European journal of oral sciences · 2026Pooled it
- Correlation between plaque control and gingival health using short and extended oral hygiene intervals.Clinical oral investigations · 2018Trial
- Normoxic stress enhance the secretion of adhesion-promoting membrane vesicles byJournal of oral microbiology · 2026Article
- Antimicrobial Activity of Ethanolic Propolis Extracts from Tame (Arauca) on Oral Biofilm Co-Cultures.Pathogens (Basel, Switzerland) · 2025Article
- Seven genome sequences ofMicrobiology resource announcements · 2025Article
- Design, development, and validation of new fluorescent strains for studying oral streptococci.Microbiology spectrum · 2025Article
- Polybacterial intracellular macromolecules shape single-cell inflammatory profiles in upper airway epithelia.NPJ biofilms and microbiomes · 2025Article
- In Vivo Study on the Salivary Kinetics of Two Probiotic Strains Delivered via Chewing Gum.Microorganisms · 2025Article
- Structure and composition of early biofilms formed on dental implants are complex, diverse, subject-specific and dynamic.NPJ biofilms and microbiomes · 2024Article
- Article
- The recovery of the microbial community after plaque removal depends on periodontal health status.NPJ biofilms and microbiomes · 2023Article
- Sensors for in situ monitoring of oral and dental health parameters in saliva.Clinical oral investigations · 2023Review
- Prevalence of Oral PathogenMicroorganisms · 2023Article
- New Technological Approaches for Dental Caries Treatment: From Liquid Crystalline Systems to Nanocarriers.Pharmaceutics · 2023Review
- Fluoride and gallein inhibit polyphosphate accumulation by oral pathogen Rothia dentocariosa.Letters in applied microbiology · 2023Article
- Preliminary landscape ofFrontiers in cellular and infection microbiology · 2023Article
- Caries Management-The Role of Surface Interactions in De- and Remineralization-Processes.Journal of clinical medicine · 2022Review
- The Common and Unique Pattern of Microbiome Profiles among Saliva, Tissue, and Stool Samples in Patients with Crohn's Disease.Microorganisms · 2022Article
- Polymicrobial Interactions of Oral Microbiota: a Historical Review and Current Perspective.mBio · 2022Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Although the mature dental biofilm composition is well studied, there is very little information on the earliest phase of in vivo tooth colonization. Progress in dental biofilm collection methodologies and techniques of large-scale microbial identification have made new studies in this field of oral biology feasible. The aim of this study was to characterize the temporal changes and diversity of the cultivable and noncultivable microbes in the early dental biofilm. Samples of early dental biofilm were collected from 11 healthy subjects at 0, 2, 4, and 6 h after removal of plaque and pellicle from tooth surfaces. With the semiquantitative Human Oral Microbiome Identification Microarray (HOMIM) technique, which is based on 16S rRNA sequence hybridizations, plaque samples were analyzed with the currently available 407 HOMIM microbial probes. This led to the identification of at least 92 species, with streptococci being the most abundant bacteria across all time points in all subjects. High-frequency detection was also made with Haemophilus parainfluenzae, Gemella haemolysans, Slackia exigua, and Rothia species. Abundance changes over time were noted for Streptococcus anginosus and Streptococcus intermedius (P = 0.02), Streptococcus mitis bv. 2 (P = 0.0002), Streptococcus oralis (P = 0.0002), Streptococcus cluster I (P = 0.003), G. haemolysans (P = 0.0005), and Stenotrophomonas maltophilia (P = 0.02). Among the currently uncultivable microbiota, eight phylotypes were detected in the early stages of biofilm formation, one belonging to the candidate bacterial division TM7, which has attracted attention due to its potential association with periodontal disease.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.