ArticleApplied and environmental microbiology2016
Evaluation of Ultrasound-Induced Damage to Escherichia coli and Staphylococcus aureus by Flow Cytometry and Transmission Electron Microscopy.
Article in Applied and environmental microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 185 citations in OpenAlex.
- Flavonoids as Potential Therapeutic Agents Against Gastrointestinal Pathogen Growth and Their Mechanisms of Action: A Comprehensive Review.Molecules (Basel, Switzerland) · 2026Review
- Evaluating Low-Frequency Ultrasound as a Pretreatment to Improve Ozonation Antimicrobial Efficacy in Urban Wastewater Treatment.Water environment research : a research publication of the Water Environment Federation · 2026Article
- Thermal Versus Ultrasound Inactivation of Bifidobacterium animalis subsp. lactis BB-12: Functional Implications for Postbiotics.Probiotics and antimicrobial proteins · 2026Article
- Metabolic Master Switch: Pyruvate Carboxylase Fuels Antimicrobial Resistance and Virulence in FoodborneFoods (Basel, Switzerland) · 2025Article
- Sonoporation-enhanced the antibacterial efficacy of aminoglycosides against Gram-negative persisters.World journal of microbiology & biotechnology · 2025Article
- Effect of Ultrasound Combined with Plasma-Activated Water on Lethal and Sublethal Injury AgainstFoods (Basel, Switzerland) · 2025Article
- Article
- Exploring ultrasound-induced metabolic attenuation inFrontiers in microbiology · 2025Article
- Flavonoids: A Review of Antibacterial Activity Against Gram-Negative Bacteria.International journal of microbiology · 2025Review
- Development and mechanism exploration of a quantitative model for Escherichia coli transformation efficiency based on ultrasonic power.Ultrasonics sonochemistry · 2024Article
- Ultrasound combined with FeSOUltrasonics sonochemistry · 2024Article
- Article
- Antibacterial Effects of Thermosonication Technology onFoods (Basel, Switzerland) · 2024Article
- Ultrasonic cavitation induced Vibrio parahaemolyticus entering an apoptosis-like death process through SOS response.Ultrasonics sonochemistry · 2024Article
- Novel methods to monitor the biodegradation of polylactic acid (PLA) byFrontiers in bioengineering and biotechnology · 2024Article
- Synergistic effect of ultrasound and antimicrobial solutions of cecropin P1 in the deactivation of Escherichia coli O157:H7 using a cylindrical ultrasonic system.Scientific reports · 2023Article
- Recent trends in the applications of sonochemical reactors as an advanced oxidation process for the remediation of microbial hazards associated with water and wastewater: A critical review.Ultrasonics sonochemistry · 2023Review
- Effective control of antibiotic resistance using a sonication-based combinational treatment and its application to fresh food.Ultrasonics sonochemistry · 2022Article
- WPI Hydrogels with a Prolonged Drug-Release Profile for Antimicrobial Therapy.Pharmaceutics · 2022Article
- Engineering microbial consortia of Elizabethkingia meningoseptica and Escherichia coli strains for the biosynthesis of vitamin K2.Microbial cell factories · 2022Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a nonthermal sterilization technique, ultrasound has attracted great interest in the field of food preservation. In this study, flow cytometry and transmission electron microscopy were employed to investigate ultrasound-induced damage to Escherichia coli and Staphylococcus aureus. For flow cytometry studies, single staining with propidium iodide (PI) or carboxyfluorescein diacetate (cFDA) revealed that ultrasound treatment caused cell death by compromising membrane integrity, inactivating intracellular esterases, and inhibiting metabolic performance. The results showed that ultrasound damage was independent of initial bacterial concentrations, while the mechanism of cellular damage differed according to the bacterial species. For the Gram-negative bacterium E. coli, ultrasound worked first on the outer membrane rather than the cytoplasmic membrane. Based on the double-staining results, we inferred that ultrasound treatment might be an all-or-nothing process: cells ruptured and disintegrated by ultrasound cannot be revived, which can be considered an advantage of ultrasound over other nonthermal techniques. Transmission electron microscopy studies revealed that the mechanism of ultrasound-induced damage was multitarget inactivation, involving the cell wall, cytoplasmic membrane, and inner structure. Understanding of the irreversible antibacterial action of ultrasound has great significance for its further utilization in the food industry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.