ArticleGenetics2016
The Female Post-Mating Response Requires Genes Expressed in the Secondary Cells of the Male Accessory Gland in Drosophila melanogaster.
Article in Genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 71 citations in OpenAlex.
- Abdominal-B regulates the male seminal fluid transferome and new fecundity factors required for sperm sex peptide binding.PLoS genetics · 2026Article
- TSSKL is essential for sperm mitochondrial morphogenesis and male fertility in moths.PLoS genetics · 2025Article
- Oncogenic signaling in the Drosophila prostate-like accessory gland activates a pro-tumorigenic program in the absence of proliferation.Disease models & mechanisms · 2025Article
- Unusual modes of cell and nuclear divisions characterise Drosophila development.Biochemical Society transactions · 2024Review
- Cell cycle variants during Drosophila male accessory gland development.G3 (Bethesda, Md.) · 2024Article
- A predominant role of genotypic variation in both expression of sperm competition genes and paternity success inProceedings. Biological sciences · 2023Article
- Review
- Viruses in LaboratoryViruses · 2023Article
- Transcriptional programs are activated and microRNAs are repressed within minutes after mating in the Drosophila melanogaster female reproductive tract.BMC genomics · 2023Article
- Article
- The evolution of sex peptide: sexual conflict, cooperation, and coevolution.Biological reviews of the Cambridge Philosophical Society · 2022Article
- CRISPR/Cas9 Mediated Disruption of Seminal Fluid Protein Sfp62 Induces Male Sterility inBiology · 2022Article
- Single-nucleus transcriptomes reveal evolutionary and functional properties of cell types in the Drosophila accessory gland.Genetics · 2022Article
- Regulation of male fertility and accessory gland gene expression by the Drosophila HR39 nuclear receptor.Developmental biology · 2021Article
- Gene expression and alternative splicing dynamics are perturbed in female head transcriptomes following heterospecific copulation.BMC genomics · 2021Article
- Identification of a micropeptide and multiple secondary cell genes that modulateProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Differences in Postmating Transcriptional Responses between Conspecific and Heterospecific Matings in Drosophila.Molecular biology and evolution · 2021Article
- ThePhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- Polyploidy in the adulteLife · 2020Article
- Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Seminal proteins from the Drosophila male accessory gland induce post-mating responses (PMR) in females. The PMR comprise behavioral and physiological changes that include increased egg laying, decreased receptivity to courting males, and changes in the storage and use of sperm. Many of these changes are induced by a "sex peptide" (SP) and are maintained by SP's binding to, and slow release from, sperm. The accessory gland contains two secretory cell types with distinct morphological and developmental characteristics. Products of these "main" and "secondary" cells work interdependently to induce and maintain the PMR. To identify individual genes needed for the morphology and function of secondary cells, we studied iab-6(cocu) males, whose secondary cells have abnormal morphology and fail to provide products to maintain the PMR. By RNA-seq, we identified 77 genes that are downregulated by a factor of >5× in iab-6(cocu) males. By functional assays and microscopy, we tested 20 candidate genes and found that at least 9 are required for normal storage and release of SP in mated females. Knockdown of each of these 9 genes consequently leads to a reduction in egg laying and an increase in receptivity over time, confirming a role for the secondary cells in maintaining the long-term PMR. Interestingly, only 1 of the 9 genes, CG3349, encodes a previously reported seminal fluid protein (Sfp), suggesting that secondary cells may perform essential functions beyond the production and modification of known Sfps. At least 3 of the 9 genes also regulate the size and/or abundance of secondary cell vacuoles, suggesting that the vacuoles' contents may be important for the machinery used to maintain the PMR.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.