ArticleJournal of cell science2016
Flagellar pocket restructuring through the Leishmania life cycle involves a discrete flagellum attachment zone.
Article in Journal of cell science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 64 citations in OpenAlex.
- The subcellular architecture ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Effects of TcFLA-1BP and TcGP72 Deletion on the Infectivity and Survival of Trypanosoma cruzi in Cell Cultures.Cell biology international · 2025Article
- Visualization of Trypanosoma brucei flagellar pocket collar biogenesis identifies two new cytoskeletal structures.PLoS biology · 2025Article
- Tubulin detyrosination shapesProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Adhesion ofmSphere · 2024Article
- Whole cell reconstructions of Leishmania mexicana through the cell cycle.PLoS pathogens · 2024Article
- Disruption of Leishmania flagellum attachment zone architecture causes flagellum loss.Molecular microbiology · 2024Article
- A sticky situation: When trypanosomatids attach to insect tissues.PLoS pathogens · 2023Review
- Implications of Flagellar Attachment Zone Proteins TcGP72 and TcFLA-1BP in Morphology, Proliferation, and Intracellular Dynamics inPathogens (Basel, Switzerland) · 2023Article
- Morphogenesis in Trypanosoma cruzi epimastigotes proceeds via a highly asymmetric cell division.PLoS neglected tropical diseases · 2023Article
- Detection and activity of MMP-2 and MMP-9 in Leishmania amazonensis and Leishmania braziliensis promastigotes.BMC microbiology · 2023Article
- ULK4 and Fused/STK36 interact to mediate assembly of a motile flagellum.Molecular biology of the cell · 2023Article
- Formation and three-dimensional architecture ofeLife · 2023Article
- New Vistas in the Biology of the Flagellum-Pathogens (Basel, Switzerland) · 2022Review
- The Trypanosomatids Cell Cycle: A Brief Report.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Cell Cycle, Telomeres, and Telomerase inCells · 2021Review
- Review
- Expansion microscopy facilitates quantitative super-resolution studies of cytoskeletal structures in kinetoplastid parasites.Open biology · 2021Article
- The kinesin of the flagellum attachment zone in Leishmania is required for cell morphogenesis, cell division and virulence in the mammalian host.PLoS pathogens · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Leishmania promastigote parasites have a flagellum, which protrudes from the flagellar pocket at the cell anterior, yet, surprisingly, have homologs of many flagellum attachment zone (FAZ) proteins--proteins used in the related Trypanosoma species to laterally attach the flagellum to the cell body from the flagellar pocket to the cell posterior. Here, we use seven Leishmania mexicana cell lines that expressed eYFP fusions of FAZ protein homologs to show that the Leishmania flagellar pocket includes a FAZ structure. Electron tomography revealed a precisely defined 3D organisation for both the flagellar pocket and FAZ, with striking similarities to those of Trypanosoma brucei. Expression of two T. brucei FAZ proteins in L. mexicana showed that T. brucei FAZ proteins can assemble into the Leishmania FAZ structure. Leishmania therefore have a previously unrecognised FAZ structure, which we show undergoes major structural reorganisation in the transition from the promastigote (sandfly vector) to amastigote (in mammalian macrophages). Morphogenesis of the Leishmania flagellar pocket, a structure important for pathogenicity, is therefore intimately associated with a FAZ; a finding with implications for understanding shape changes involving component modules during evolution.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.