ArticleMolecular brain2016
ASIC subunit ratio and differential surface trafficking in the brain.
Article in Molecular brain, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- The multifaceted role of ASICs channels into the nervous system and their potential use as a therapeutic target for neuro-disorders.Journal of neural transmission (Vienna, Austria : 1996) · 2026Review
- Acid-sensing ion channel 2a assembles with epithelial NaThe Journal of biological chemistry · 2026Article
- Gene arrangement within concatemeric constructs governs the functional divergence of human and rat ASIC3 concatemers.Journal of physiology and biochemistry · 2026Article
- Acid-sensing ion channels and their role in controlling pain in the post-operative stage.International journal of physiology, pathophysiology and pharmacology · 2026Review
- The secreted protein PCYOX1L controls the surface expression of acid-sensing ion channel 1a.Science advances · 2025Article
- The Role of Acid-Sensing Ion Channel 1A (ASIC1A) in the Behavioral and Synaptic Effects of Oxycodone and Other Opioids.International journal of molecular sciences · 2024Article
- Review
- Enhanced harm detection following maternal separation: Transgenerational transmission and reversibility by inhaled amiloride.Science advances · 2023Article
- Functional characterization of acid-sensing ion channels in the cerebellum-originating medulloblastoma cell line DAOY and in cerebellar granule neurons.Pflugers Archiv : European journal of physiology · 2023Article
- Molecular and biophysical features of hippocampal "lipid rafts aging" are modified by dietary n-3 long-chain polyunsaturated fatty acids.Aging cell · 2023Article
- Physiologically relevant acid-sensing ion channel (ASIC) 2a/3 heteromers have a 1:2 stoichiometry.Communications biology · 2023Article
- Anxiolytic, Analgesic and Anti-Inflammatory Effects of Peptides Hmg 1b-2 and Hmg 1b-4 from the Sea AnemoneToxins · 2023Article
- Intoxicating effects of alcohol depend on acid-sensing ion channels.Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology · 2023Article
- Aggressive migration in acidic pH of a glioblastoma cancer stem cell line in vitro is independent of ASIC and KPflugers Archiv : European journal of physiology · 2023Article
- Investigating role of ASIC2 in synaptic and behavioral responses to drugs of abuse.Frontiers in molecular biosciences · 2023Article
- Dynamic Distribution of ASIC1a Channels and Other Proteins within Cells Detected through Fractionation.Membranes · 2022Article
- Acid-Sensing Ion Channel 2: Function and Modulation.Membranes · 2022Review
- Extracellular Protons Mediate Presynaptic Homeostatic Potentiation at the Mouse Neuromuscular Junction.Neuroscience · 2021Article
- Acid-Sensing Ion Channels and Mechanosensation.International journal of molecular sciences · 2021Review
- Signaling Pathways in Proton and Non-proton ASIC1a Activation.Frontiers in cellular neuroscience · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
backgroundAcid-sensing ion channels (ASICs) are key mediators of acidosis-induced responses in neurons. However, little is known about the relative abundance of different ASIC subunits in the brain. Such data are fundamental for interpreting the relative contribution of ASIC1a homomers and 1a/2 heteromers to acid signaling, and essential for designing therapeutic interventions to target these channels. We used a simple biochemical approach and semi-quantitatively determined the molar ratio of ASIC1a and 2 subunits in mouse brain. Further, we investigated differential surface trafficking of ASIC1a, ASIC2a, and ASIC2b. RESULTS AND
conclusionsASIC1a subunits outnumber the sum of ASIC2a and ASIC2b. There is a region-specific variation in ASIC2a and 2b expression, with cerebellum and striatum expressing predominantly 2b and 2a, respectively. Further, we performed surface biotinylation and found that surface ASIC1a and ASIC2a ratio correlates with their total expression. In contrast, ASIC2b exhibits little surface presence in the brain. This result is consistent with increased co-localization of ASIC2b with an ER marker in 3T3 cells. Our data are the first semi-quantitative determination of relative subunit ratio of various ASICs in the brain. The differential surface trafficking of ASICs suggests that the main functional ASICs in the brain are ASIC1a homomers and 1a/2a heteromers. This finding provides important insights into the relative contribution of various ASIC complexes to acid signaling in neurons.
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