ArticleTicks and tick-borne diseases2016
Target validation of highly conserved Amblyomma americanum tick saliva serine protease inhibitor 19.
Article in Ticks and tick-borne diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- In silico screening and immunogenic features of putative tick cement protein PA107 from Ixodes ricinus tick.Experimental & applied acarology · 2025Article
- Serpins in the Spotlight: Novel Bioinformatic Insights IntoBiochemistry research international · 2025Article
- Binding Molecules in Tick Saliva for Targeting Host Cytokines, Chemokines, and Beyond.Biomolecules · 2024Review
- In vitro identification of neutralizing epitopes of Rhipicephalus microplus serpin 17 (RmS-17).Vaccine · 2024Article
- Immune protection of three serine protease inhibitors vaccine in mice against Rhipicephalus sanguineus.Scientific reports · 2024Article
- A tick saliva serpin, IxsS17 inhibits host innate immune system proteases and enhances host colonization by Lyme disease agent.PLoS pathogens · 2024Article
- Key molecules regulating the blood meals ofVeterinary research forum : an international quarterly journal · 2024Article
- Rabbits as Animal Models for Anti-Tick Vaccine Development: A Global Scenario.Pathogens (Basel, Switzerland) · 2023Review
- Recent Advances in Tick Antigen Discovery and Anti-Tick Vaccine Development.International journal of molecular sciences · 2023Review
- Article
- Serpins in Tick Physiology and Tick-Host Interaction.Frontiers in cellular and infection microbiology · 2022Review
- rDromaserpin: A Novel Anti-Hemostatic Serpin, from the Salivary Glands of the Hard TickToxins · 2021Article
- Article
- Borrelia burgdorferi infection modifies protein content in saliva of Ixodes scapularis nymphs.BMC genomics · 2021Article
- Iripin-3, a New Salivary Protein Isolated FromFrontiers in immunology · 2021Article
- The immunosuppressive functions of two novel tick serpins, HlSerpin-a and HlSerpin-b, from Haemaphysalis longicornis.Immunology · 2020Article
- Amblyomma americanum serpin 27 (AAS27) is a tick salivary anti-inflammatory protein secreted into the host during feeding.PLoS neglected tropical diseases · 2019Article
- Immunomodulatory effects of Rhipicephalus haemaphysaloides serpin RHS2 on host immune responses.Parasites & vectors · 2019Article
- Disruption of blood meal-responsive serpins prevents Ixodes scapularis from feeding to repletion.Ticks and tick-borne diseases · 2018Article
- Identification and characterization of proteins in the Amblyomma americanum tick cement cone.International journal for parasitology · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Amblyomma americanum tick serine protease inhibitor (serpin, AAS) 19, is a highly conserved protein that is characterized by its functional domain being 100% conserved across tick species. We also reported that AAS19 was an immunogenic tick saliva protein with anti-haemostatic functions and an inhibitor of trypsin-like proteases including five of the eight serine protease factors in the blood clotting cascade. In this study the goal was to validate the importance of AAS19 in A. americanum tick physiology, assess immunogenicity and investigate tick vaccine efficacy of yeast-expressed recombinant (r) AAS19. We confirm that AAS19 is important to A. americanum fitness and blood meal feeding. AAS19 mRNA disruption by RNAi silencing caused ticks to obtain blood meals that were 50% smaller than controls, and treated ticks being morphologically deformed with 100% of the deformed ticks dying in incubation. We show that rAAS19 is highly immunogenic in that two 500 μg inoculations mixed with TiterMax Gold adjuvant provoked antibody titers of more than 1:320,000 that specifically reacted with native AAS19 in unfed and partially fed tick tissue. Since AAS19 is injected into animals during tick feeding, we challenge infested immunized rabbits twice to test if tick infestations of immunized rabbits could act as booster. While in the first infestation significantly smaller tick blood meals were observed on one of the two immunized rabbits, smaller blood meals were observed on both rabbits, but 60% of ticks that engorged on immunized rabbits in the second infestation failed to lay eggs. It is notable that ticks fed faster on immunized animals despite obtaining smaller blood meals. We conclude that rAAS19 is a potential component of cocktail tick vaccine.
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