Evidence map›Paper›PMID 26746047›Full record

ReviewAdvances in biological regulation2016

Phospholipase Cβ connects G protein signaling with RNA interference.

Suzanne Scarlata, Osama Garwain, Leo Williams, Imanol Gonzalez Burguera, Barbara Rosati, Shriya Sahu, Yuanjian Guo, Finly Philip, Urszula Golebiewska

Open access · greenAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Advances in biological regulation, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Review
  3. Molecular regulation of PLCβ signaling.Methods in enzymology · 2023
    Review
  4. Phospholipase C.Advances in experimental medicine and biology · 2020
    Review
  5. Review
  6. Phospholipase Cβ1 regulates proliferation of neuronal cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018
    Article
  7. Review
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Suzanne ScarlataDept. of Physiology & Biophysics, Stony Brook University, Stony Brook, NY 11794-8661, USA; Dept. of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA. Electronic address: sfscarlata@wpi.edu.
Osama GarwainDept. of Physiology & Biophysics, Stony Brook University, Stony Brook, NY 11794-8661, USA; Dept. of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Leo WilliamsDept. of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Imanol Gonzalez BurgueraDept. of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA; Departamento de Neurociencias, Universidad del País Vasco (UPV/EHU), Spain.
Barbara RosatiDept. of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Shriya SahuDept. of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Yuanjian GuoDept. of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Finly PhilipDept. of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Urszula GolebiewskaDept. of Chemistry and Biochemistry, Worcester Polytechnic Institute, Worcester, MA 01609, USA; Dept of Biological Sciences and Geology, Queensborough Community College, Bayside, NY 11364, USA.
Worcester Polytechnic Institute · US

Funding

Role of caveolae in G protein signalingR01GM116178 · NIGMS · WORCESTER POLYTECHNIC INSTITUTE · PI SCARLATA, SUZANNE F · 2015 to 2018
$1.1M
NIGMS NIH HHS R01 GM116178
6 · The paper itself

Abstract

Phosphoinositide-specific-phospholipase Cβ (PLCβ) is the main effector of Gαq stimulation which is coupled to receptors that bind acetylcholine, bradykinin, dopamine, angiotensin II as well as other hormones and neurotransmitters. Using a yeast two-hybrid and other approaches, we have recently found that the same region of PLCβ that binds Gαq also interacts with Component 3 Promoter of RNA induced silencing complex (C3PO), which is required for efficient activity of the RNA-induced silencing complex. In purified form, C3PO competes with Gαq for PLCβ binding and at high concentrations can quench PLCβ activation. Additionally, we have found that the binding of PLCβ to C3PO inhibits its nuclease activity leading to reversal of RNA-induced silencing of specific genes. In cells, we found that PLCβ distributes between the plasma membrane where it localizes with Gαq, and in the cytosol where it localizes with C3PO. When cells are actively processing small interfering RNAs the interaction between PLCβ and C3PO gets stronger and leads to changes in the cellular distribution of PLCβ. The magnitude of attenuation is specific for different silencing RNAs. Our studies imply a direct link between calcium responses mediated through Gαq and post-transcriptional gene regulation through PLCβ.

Indexed as

RNA InterferenceAnimalsBinding, CompetitiveBinding SitesCalciumCell MembraneCytosolGTP-Binding Protein alpha Subunits, Gq-G11HEK293 CellsHumansPhospholipase C betaProtein BindingRNA-Induced Silencing ComplexRNA, Small InterferingSignal TransductionCalciumGTP-Binding Protein alpha Subunits, Gq-G11Phospholipase C betaRNA-Induced Silencing ComplexRNA, Small InterferingCalcium signalingComponent 3 promoter of RNA silencingG protein signalingPhospholipase CβRNA silencing

Identifiers

PMID26746047
PMCPMC4884482
OpenAlexW2185332337

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.