ArticleWorld journal of microbiology & biotechnology2016
β-1,3-1,4-glucanase gene from Bacillus velezensis ZJ20 exerts antifungal effect on plant pathogenic fungi.
Article in World journal of microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 70 citations in OpenAlex.
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- Whole genome-sequence analysis of Bacillus subtilis strain KC14-1 with broad-spectrum antifungal activity.BMC genomics · 2025Article
- Controlling mildew of tobacco leaf by Bacillus amyloliquefaciens ZH-2 and its effect on storage quality of tobacco leaf.Scientific reports · 2025Article
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- Biological Control of Fusarium oxysporum, the Causal Agent of Fusarium Basal Rot in Onion by Bacillus spp.The plant pathology journal · 2023Article
- The Prospect of Hydrolytic Enzymes fromInternational journal of molecular sciences · 2023Review
- Purification, characterization, immobilization and applications of an enzybiotic β-1,3-1,4-glucanase produced from halotolerant marine Halomonas meridiana ES021.World journal of microbiology & biotechnology · 2023Article
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- Biocontrol ability ofFrontiers in microbiology · 2023Article
- Stimulating fungal cell wall integrity by exogenous β-glucanase to improve the production of fungal natural products.Applied microbiology and biotechnology · 2022Article
- Antifungal activity and genomic characterization of the biocontrol agent Bacillus velezensis CMRP 4489.Scientific reports · 2022Article
- Evaluation of the Antifungal Activity ofBiology · 2022Article
- Comparative Genome Analysis Reveals Phylogenetic Identity of Bacillus velezensis HNA3 and Genomic Insights into Its Plant Growth Promotion and Biocontrol Effects.Microbiology spectrum · 2022Article
- Induction of Systemic Resistance in Maize and Antibiofilm Activity of Surfactin FromFrontiers in microbiology · 2022Article
- Genomic Analysis Reveals Potential Mechanisms Underlying Promotion of Tomato Plant Growth and Antagonism of Soilborne Pathogens by Bacillus amyloliquefaciens Ba13.Microbiology spectrum · 2021Article
- Review
- Beneficial Microorganisms to Control the Gray Mold of Grapevine: From Screening to Mechanisms.Microorganisms · 2021Article
- Enhanced cercosporin production by co-culturing Cercospora sp. JNU001 with leaf-spot-disease-related endophytic bacteria.Microbial cell factories · 2021Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacillus velezensis is a known antifungal bacteria. To understand the role of β-1, 3-1, 4-glucanase played on B. velezensis about the mechanism which exerts effect on fungi, we isolated and cloned the β-1, 3-1, 4-glucanase gene (Bglu1) from B. velezensis ZJ20. The Bglu1 open reading frame was 732 bp that encoded a protein with 243 amino acids and a calculated molecular weight of 27.3 kDa. The same gene without the signal peptide, termed Bglu2, was also cloned and expressed in E. coli BL21. Among the two variants, only Bglu2 protein was expressed. Purified Bglu2 could be eluted with imidazole solution at concentrations ranging from 100 to 500 mM although the highest expression was observed at 150 and 200 mM and the purest was at 500 mM. In addition, activity of the crude enzyme was 1527 U ml(-1) and the highest activity of the purified enzyme was 1706 U ml(-1). The purified β-1, 3-1, 4-glucanase had activity on a wide range of pH and temperatures and displayed optimal activity at pH 5.0 and 35 °C. More importantly, the mycelial morphology of three pathogenic fungi was destroyed by the purified β-1, 3-1, 4-glucanase. In conclusion, β-1, 3-1, 4-glucanase from B. velezensis ZJ20 can be highly expressed in E. coli BL21 and the recombinant protein is pathogenic to fungi.
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