ArticlePloS one2016
Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata).
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
30 citing papers in PubMed, 56 citations in OpenAlex.
- In-vitro and In-silico Evaluation of Supercritical Extract of Acacia Auriculiformis against Breast Cancer.Cell biochemistry and biophysics · 2026Article
- Phytochemical Screening and Physicochemical Properties of Oil Extract ofInternational journal of molecular sciences · 2026Article
- Methylated 1,2-naphthoquinone derivative SJ006 as an inhibitor of human glucose 6-phosphate dehydrogenase in non-small cell lung cancer cell lines.Scientific reports · 2025Article
- Comprehensive Phytochemical Analysis and Evaluation of Antioxidant, Antimicrobial, Cytotoxic, and Immunomodulatory Activities of Commercial Cinnamon Bark Essential Oil (International journal of molecular sciences · 2025Article
- Autophagy as a targeted therapeutic approach for skin cancer: Evaluating natural and synthetic molecular interventions.Cancer pathogenesis and therapy · 2024Review
- Article
- Methodological Aspects of Green Extraction of Usnic Acid Using Natural Deep Eutectic Solvents.Molecules (Basel, Switzerland) · 2023Article
- Inhibition of Autophagy Increases Cell Death in HeLa Cells through Usnic Acid Isolated from Lichens.Plants (Basel, Switzerland) · 2023Article
- ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma byInternational journal of molecular sciences · 2022Article
- Design, Characterization, and Anticancer and Antimicrobial Activities of Mucoadhesive Oral Patches Loaded withAntioxidants (Basel, Switzerland) · 2022Article
- Methanol Extract ofAntioxidants (Basel, Switzerland) · 2022Article
- Evaluation ofAntioxidants (Basel, Switzerland) · 2022Article
- Quatsomes Formulated with l-Prolinol-Derived Surfactants as Antibacterial Nanocarriers of (+)-Usnic Acid with Antioxidant Activity.ACS applied nano materials · 2022Article
- Antioxidant, Cytotoxic, and Rheological Properties of Canola Oil Extract ofPlants (Basel, Switzerland) · 2022Article
- Review
- Usnic Acid andAntioxidants (Basel, Switzerland) · 2021Article
- In Vitro Anticancer Activity and Oxidative Stress Biomarkers Status Determined byAntioxidants (Basel, Switzerland) · 2021Article
- Antioxidant and Cytotoxic Activities ofPlants (Basel, Switzerland) · 2021Article
- Microwave-Assisted Extraction and HPLC-UV-CD Determination of (S)-usnic Acid inMolecules (Basel, Switzerland) · 2021Article
- Evaluation of the Anti-Inflammatory and Anti-Oxidative Effects of Therapeutic Human Lactoferrin Fragments.Frontiers in bioengineering and biotechnology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/or in oxidative stress-mediated conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.