ArticleAnalytical chemistry2016
A Microfluidic Device with Integrated Sonication and Immunoprecipitation for Sensitive Epigenetic Assays.
Article in Analytical chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Advancements in Sonication-Based Extraction Techniques for Ovarian Follicular Fluid Analysis: Implications for Infertility Diagnostics and Assisted Reproductive Technologies.International journal of molecular sciences · 2025Review
- Microfluidic systems and ultrasonics for emulsion-based biopolymers: A comprehensive review of techniques, challenges, and future directions.Ultrasonics sonochemistry · 2025Review
- A Bi-Directional Acoustic Micropump Driven by Oscillating Sharp-Edge Structures.Micromachines · 2023Article
- Microfluidic Continuous Flow DNA Fragmentation based on a Vibrating Sharp-tip.Microfluidics and nanofluidics · 2022Article
- Epigenetic Mechanisms as Emerging Therapeutic Targets and Microfluidic Chips Application in Pulmonary Arterial Hypertension.Biomedicines · 2022Review
- Ultrasound-assisted production and optimization of mini-emulsions in a microfluidic chip in continuous-flow.Ultrasonics sonochemistry · 2021Article
- Acoustic Streaming Generated by Sharp Edges: The Coupled Influences of Liquid Viscosity and Acoustic Frequency.Micromachines · 2020Article
- Translational Opportunities for Microfluidic Technologies to Enable Precision Epigenomics.Analytical chemistry · 2020Article
- Perspectives on the Role of Histone Modification in Breast Cancer Progression and the Advanced Technological Tools to Study Epigenetic Determinants of Metastasis.Frontiers in genetics · 2020Review
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- Microfluidic MeDIP-seq for low-input methylomic analysis of mammary tumorigenesis in mice.The Analyst · 2019Article
- Microfluidic Low-Input Fluidized-Bed Enabled ChIP-seq Device for Automated and Parallel Analysis of Histone Modifications.Analytical chemistry · 2018Article
- Low-input and multiplexed microfluidic assay reveals epigenomic variation across cerebellum and prefrontal cortex.Science advances · 2018Article
- Microfluidics for genome-wide studies involving next generation sequencing.Biomicrofluidics · 2017Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Epigenetic studies increasingly require analysis of a small number of cells that are of one specific type and derived from patients or animals. In this report, we demonstrate a simple microfluidic device that integrates sonication and immunoprecipitation (IP) for epigenetic assays, such as chromatin immunoprecipitation (ChIP) and methylated DNA immunoprecipitation (MeDIP). By incorporating an ultrasonic transducer with a microfluidic chamber, we implemented microscale sonication for both shearing chromatin/DNA and mixing/washing of IP beads. Such integration allowed highly sensitive tests starting with 100 cross-linked cells for ChIP or 500 pg of genomic DNA for MeDIP (compared to 10(6)-10(7) cells for ChIP and 1-10 μg of DNA for MeDIP in conventional assays). The entire on-chip process of sonication and IP took only 1 h. Our tool will be useful for highly sensitive epigenetic studies based on a small quantity of sample.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.