ArticleBiophysical journal2016
Label-free Imaging of Microtubules with Sub-nm Precision Using Interferometric Scattering Microscopy.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Multi-camera Simultaneous Total Internal Reflection and Interference Reflection Microscopy.bioRxiv : the preprint server for biology · 2024Article
- Real-Time Monitoring and Control of Nanoparticle Formation.Journal of the American Chemical Society · 2023Article
- A photonic resonator interferometric scattering microscope for label-free detection of nanometer-scale objects with digital precision in point-of-use environments.Biosensors & bioelectronics · 2023Article
- Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells.Nature communications · 2023Article
- A Photonic Resonator Interferometric Scattering Microscope for Label-free Detection of Nanometer-Scale Objects with Digital Precision in Point-of-Use Environments.bioRxiv : the preprint server for biology · 2022Article
- Analysis tools for single-monomer measurements of self-assembly processes.Scientific reports · 2022Article
- Quantification of microtubule stutters: dynamic instability behaviors that are strongly associated with catastrophe.Molecular biology of the cell · 2022Article
- Zero-mode waveguides visualize the first steps during gelsolin-mediated actin filament formation.Biophysical journal · 2022Article
- Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.Chemical reviews · 2021Review
- Integrated multi-wavelength microscope combining TIRFM and IRM modalities for imaging cellulases and other processive enzymes.Biomedical optics express · 2021Article
- Molecular mechanisms underlying microtubule growth dynamics.Current biology : CB · 2021Review
- Polycationic gold nanorods as multipurposeNanoscale advances · 2020Article
- The mechanochemistry of the kinesin-2 KIF3AC heterodimer is related to strain-dependent kinetic properties of KIF3A and KIF3C.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- In Vitro Microtubule Dynamics Assays Using Dark-Field Microscopy.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules.Journal of visualized experiments : JoVE · 2019Article
- Interferometric Scattering Enables Fluorescence-Free Electrokinetic Trapping of Single Nanoparticles in Free Solution.Nano letters · 2019Article
- Direct observation of individual tubulin dimers binding to growing microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Single-Nanoparticle Tracking with Angstrom Localization Precision and Microsecond Time Resolution.Biophysical journal · 2018Article
- Label-free high-speed wide-field imaging of single microtubules using interference reflection microscopy.Journal of microscopy · 2018Article
- Label-free Imaging and Bending Analysis of Microtubules by ROCS Microscopy and Optical Trapping.Biophysical journal · 2018Article
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
5 authors.
Funding
Abstract
Current in vitro optical studies of microtubule dynamics tend to rely on fluorescent labeling of tubulin, with tracking accuracy thereby limited by the quantum yield of fluorophores and by photobleaching. Here, we demonstrate label-free tracking of microtubules with nanometer precision at kilohertz frame rates using interferometric scattering microscopy (iSCAT). With microtubules tethered to a glass substrate using low-density kinesin, we readily detect sequential 8 nm steps in the microtubule center of mass, characteristic of a single kinesin molecule moving a microtubule. iSCAT also permits dynamic changes in filament length to be measured with <5 nm precision. Using the arbitrarily long observation time enabled by label-free iSCAT imaging, we demonstrate continuous monitoring of microtubule disassembly over a 30 min period. The ability of iSCAT to track microtubules with nm precision together with its potential for label-free single protein detection and simultaneous single molecule fluorescence imaging represent a unique platform for novel approaches to studying microtubule dynamics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.