ArticleBiophysical journal2016
Determinants of Cx43 Channel Gating and Permeation: The Amino Terminus.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Lipids intercalate and mediate multi-channel assemblies of connexin-46/50 gap junctions.bioRxiv : the preprint server for biology · 2026Article
- Research progress of connexins in epileptogensis.Acta epileptologica · 2025Review
- Genetically engineered human embryonic kidney cells as a novel vehicle for dual patch clamp study of human gap junction channels.The Biochemical journal · 2024Article
- Simulation of gap junction formation reveals critical role of Cys disulfide redox state in connexin hemichannel docking.Cell communication and signaling : CCS · 2024Article
- Estradiol and 3β-diol protect female cortical astrocytes by regulating connexin 43 Gap Junctions.Molecular and cellular endocrinology · 2023Article
- Patch clamp studies on TRPV4-dependent hemichannel activation in lens epithelium.Frontiers in pharmacology · 2023Article
- The Anti-Epileptic Effects of Carbenoxolone In Vitro and In Vivo.International journal of molecular sciences · 2022Article
- Connexin 43 Gene Ablation Does Not Alter Human Pluripotent Stem Cell Germ Lineage Specification.Biomolecules · 2021Article
- Review
- Gap19, a Cx43 Hemichannel Inhibitor, Acts as a Gating Modifier That Decreases Main State Opening While Increasing Substate Gating.International journal of molecular sciences · 2020Article
- Upregulated Connexin 43 Induced by Loss-of-Functional S284L-Mutant α4 Subunit of Nicotinic ACh Receptor Contributes to Pathomechanisms of Autosomal Dominant Sleep-Related Hypermotor Epilepsy.Pharmaceuticals (Basel, Switzerland) · 2020Article
- Calmodulin-Mediated Regulation of Gap Junction Channels.International journal of molecular sciences · 2020Review
- The Multifaceted Role of Astrocyte Connexin 43 in Ischemic Stroke Through Forming Hemichannels and Gap Junctions.Frontiers in neurology · 2020Review
- Connexins-Based Hemichannels/Channels and Their Relationship with Inflammation, Seizures and Epilepsy.International journal of molecular sciences · 2019Review
- The connexin26 human mutation N14K disrupts cytosolic intersubunit interactions and promotes channel opening.The Journal of general physiology · 2019Article
- Cx43 Channel Gating and Permeation: Multiple Phosphorylation-Dependent Roles of the Carboxyl Terminus.International journal of molecular sciences · 2018Article
- Regulation of Cx37 channel and growth-suppressive properties by phosphorylation.Journal of cell science · 2017Article
- The NHAmerican journal of physiology. Cell physiology · 2017Article
- Mechanism of gating by calcium in connexin hemichannels.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Specific functional pathologies of Cx43 mutations associated with oculodentodigital dysplasia.Molecular biology of the cell · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Separate connexin domains partake in proposed gating mechanisms of gap junction channels. The amino-terminus (NT) domains, which contribute to voltage sensing, may line the channel's cytoplasmic-facing funnel surface, stabilize the channel's overall structure through interactions with the transmembrane domains and each other, and integrate to form a compound particle to gate the channel closed. Interactions of the carboxyl-terminus (CT) and cytoplasmic loop (CL) domains underlie voltage- and low pH-triggered channel closure. To elucidate potential cooperation of these gating mechanisms, we replaced the Cx43NT with the Cx37NT (chimera Cx43(∗)NT37), leaving the remainder of the Cx43 sequence, including the CT and CL, unchanged. Compared to wild-type Cx43 (Cx43WT), Cx43(∗)NT37 junctions exhibited several functional alterations: extreme resistance to halothane- and acidification-induced uncoupling, absence of voltage-dependent fast inactivation, longer channel open times, larger unitary channel conductances, low junctional dye permeability/permselectivity, and an overall cation selectivity more typical of Cx37WT than Cx43WT junctions. Together, these results suggest a cohesive model of channel function wherein: 1) channel conductance and size selectivity are largely determined by pore diameter, whereas charge selectivity results from the NT domains, and 2) transition between fully open and (multiple) closed states involves global changes in structure of the pore-forming domains transduced by interactions of the pore-forming domains with either the NT, CT, or both, with the NT domains forming the gate of the completely closed channel.
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