ArticleProceedings of the National Academy of Sciences of the United States of America2016
Hairy and Groucho mediate the action of juvenile hormone receptor Methoprene-tolerant in gene repression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed, 69 citations in OpenAlex.
- Genetic and Molecular Regulation of Ecdysone Biosynthesis During Insect Metamorphosis.Insects · 2026Review
- Juvenile hormone-mediated chromatin remodelling by cumulative action of downstream transcription factors, Hairy and Krüppel homolog 1.Communications biology · 2026Article
- Two neuropeptides that promote blood feeding ineLife · 2026Article
- Blood feeding, microbiota, and layered mosquito immunity: from pathway phenotypes to vector competence.Frontiers in immunology · 2026Review
- Membrane-localized MET engages PVR to mediate extranuclear juvenile hormone signaling inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Methoprene-Tolerant (Met) Acts as Methyl Farnesoate Receptor to Regulate Larva Metamorphosis in Mud Crab,International journal of molecular sciences · 2024Article
- Schlank orchestrates insect developmental transition by switching H3K27 acetylation to trimethylation in the prothoracic gland.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Leucine aminopeptidase1 controls egg deposition and hatchability in male Aedes aegypti mosquitoes.Nature communications · 2024Article
- Identification of CYCLE targets that contribute diverse features of circadian rhythms in the mosquito Culex pipiens.Comparative biochemistry and physiology. Part D, Genomics & proteomics · 2023Article
- Using machine learning to predict protein-protein interactions between a zombie ant fungus and its carpenter ant host.Scientific reports · 2023Article
- Juvenile hormone receptor Methoprene tolerant: Functions and applications.Vitamins and hormones · 2023Article
- Knockdown ofBiology · 2022Article
- Effects of Juvenile Hormone Analog and Days after Emergence on the Reproduction of Oriental Armyworm,Insects · 2022Article
- Direct and indirect gene repression by the ecdysone cascade during mosquito reproductive cycle.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Role ofInternational journal of molecular sciences · 2022Article
- Article
- Krüppel-homolog 1 exerts anti-metamorphic and vitellogenic functions in insects via phosphorylation-mediated recruitment of specific cofactors.BMC biology · 2021Article
- Histone H3K27 methylation-mediated repression ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Juvenile hormone regulation of microRNAs is mediated by E75 in the Dengue vector mosquitoProceedings of the National Academy of Sciences of the United States of America · 2021Article
- E93 confers steroid hormone responsiveness of digestive enzymes to promote blood meal digestion in the midgut of the mosquito Aedes aegypti.Insect biochemistry and molecular biology · 2021Article
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10 authors at 3 institutions in 2 countries.
Funding
Abstract
The arthropod-specific juvenile hormone (JH) controls numerous essential functions. Its involvement in gene activation is known to be mediated by the transcription factor Methoprene-tolerant (Met), which turns on JH-controlled genes by directly binding to E-box-like motifs in their regulatory regions. However, it remains unclear how JH represses genes. We used the Aedes aegypti female mosquito, in which JH is necessary for reproductive maturation, to show that a repressor, Hairy, is required for the gene-repressive action of JH and Met. The RNA interference (RNAi) screen for Met and Hairy in the Aedes female fat body revealed a large cohort of Met- and Hairy-corepressed genes. Analysis of selected genes from this cohort demonstrated that they are repressed by JH, but RNAi of either Met or Hairy renders JH ineffective in repressing these genes in an in vitro fat-body culture assay. Moreover, this JH action was prevented by the addition of the translational inhibitor cycloheximide (CHX) to the culture, indicating the existence of an indirect regulatory hierarchy. The lack of Hairy protein in the CHX-treated tissue was verified using immunoblot analysis, and the upstream regions of Met/Hairy-corepressed genes were shown to contain common binding motifs that interact with Hairy. Groucho (gro) RNAi silencing phenocopied the effect of Hairy RNAi knockdown, indicating that it is involved in the JH/Met/Hairy hierarchy. Finally, the requirement of Hairy and Gro for gene repression was confirmed in a cell transfection assay. Thus, our study has established that Hairy and its cofactor Gro mediate the repressive function of JH and Met.
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