ArticlePlanta2016
Identification of a novel hedycaryol synthase gene isolated from Camellia brevistyla flowers and floral scent of Camellia cultivars.
Article in Planta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Coloration differences in three Camellia reticulata Lindl. cultivars: 'Tongzimian', 'Shizitou' and 'Damanao'.BMC plant biology · 2024Article
- Molecular mechanisms underlying floral fragrance inFrontiers in plant science · 2024Article
- Metabolomics Analysis Reveals the Accumulation Patterns of Flavonoids and Volatile Compounds inMolecules (Basel, Switzerland) · 2023Article
- Hedycaryol - Central Intermediates in Sesquiterpene Biosynthesis, Part II.Chemistry (Weinheim an der Bergstrasse, Germany) · 2022Review
- Engineering of Ancestors as a Tool to Elucidate Structure, Mechanism, and Specificity of Extant Terpene Cyclase.Journal of the American Chemical Society · 2021Article
- Terpene Synthases and Terpene Variation inPlant physiology · 2020Article
- The complete functional characterisation of the terpene synthase family in tomato.The New phytologist · 2020Article
- Characterization of the complete chloroplast genome ofMitochondrial DNA. Part B, Resources · 2020Article
- Gene Networks Underlying Cannabinoid and Terpenoid Accumulation in Cannabis.Plant physiology · 2019Article
- Identification of novel sesquiterpene synthase genes that mediate the biosynthesis of valerianol, which was an unknown ingredient of tea.Scientific reports · 2018Article
- Direct production of dihydroxylated sesquiterpenoids by a maize terpene synthase.The Plant journal : for cell and molecular biology · 2018Article
- Genetic diversity, population structure, and traditional culture ofEcology and evolution · 2017Article
- Structural and Chemical Biology of Terpenoid Cyclases.Chemical reviews · 2017Review
- Transcriptome Sequencing Analysis Reveals a Difference in Monoterpene Biosynthesis between ScentedFrontiers in plant science · 2017Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionA novel terpene synthase (Tps) gene isolated from Camellia brevistyla was identified as hedycaryol synthase, which was shown to be expressed specifically in flowers. Camellia plants are very popular because they bloom in winter when other plants seldom flower. Many ornamental cultivars of Camellia have been bred mainly in Japan, although the fragrance of their flowers has not been studied extensively. We analyzed floral scents of several Camellia cultivars by gas chromatography-mass spectrometry (GC-MS) and found that Camellia brevistyla produced various sesquiterpenes in addition to monoterpenes, whereas Camellia japonica and its cross-lines produced only monoterpenes, including linalool as the main product. From a flower of C. brevistyla, we isolated one cDNA encoding a terpene synthase (TPS) comprised of 554 amino acids, which was phylogenetically positioned to a sole gene clade. The cDNA, designated CbTps1, was expressed in mevalonate-pathway-engineered Escherichia coli, which carried the Streptomyces mevalonate-pathway gene cluster in addition to the acetoacetate-CoA ligase gene. A terpene product was purified from recombinant E. coli cultured with lithium acetoacetate, and analyzed by (1)H-nulcear magnetic resonance spectroscopy ((1)H-NMR) and GC-MS. It was shown that a sesquiterpene hedycaryol was produced, because (1)H-NMR signals of the purified product were very broad, and elemol, a thermal rearrangement product from hedycaryol, was identified by GC-MS analysis. Spectroscopic data of elemol were also determined. These results indicated that the CbTps1 gene encodes hedycaryol synthase. Expression analysis of CbTps1 showed that it was expressed specifically in flowers, and hedycaryol is likely to be one of the terpenes that attract insects for pollination of C. brevistyla. A linalool synthase gene, which was isolated from a flower of Camellia saluenensis, is also described.
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