ArticleThe Journal of biological chemistry2016
Conserved Amphipathic Helices Mediate Lipid Droplet Targeting of Perilipins 1-3.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed.
- Association of the PLIN1 Amphipathic Helix with Membrane Mimetics Shows a Threshold-like Dependence on Phosphatidylglycerol Content.The Journal of membrane biology · 2026Article
- Mechanisms influencing transient cytoplasmic protein targeting to intracellular lipid droplets.Biochemical Society transactions · 2026Review
- A conserved sequence insert of fibrillins is involved in plastoglobule association and lipid binding.Plant & cell physiology · 2026Article
- Macrophage-specific N-terminal truncation of Plin2 limits the size of lipid droplets.The Biochemical journal · 2026Article
- Intracrine FFA4 signaling controls lipolysis at lipid droplets.Nature chemical biology · 2026Article
- PLIN3: a multifaceted regulator of lipid droplet dynamics and disease pathogenesis.Medical oncology (Northwood, London, England) · 2025Review
- DDHD2 possesses both lipase and transacylase capacities that remodel triglyceride acyl chains.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Navigating lipid droplet proteins - part II: molecular mechanisms underlying ER-to-lipid droplet protein partitioning.Biochemical Society transactions · 2025Review
- Targeting Seipin to Alleviate Hepatic Steatosis in Zebrafish (Danio Rerio).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Quantifying the Thresholds of the Phospholipid Surface Density for Nonspecific Protein Adsorption and Desorption at the Triacylglycerol/Water Interface.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- DDHD2 possesses both lipase and transacylase capacities that remodel triglyceride acyl chains.bioRxiv : the preprint server for biology · 2025Article
- Essential Biology of Lipid Droplets.Annual review of biochemistry · 2025Review
- The C-terminal end of PLIN1 displays structural disorder.Biochemistry and biophysics reports · 2025Article
- Molecular determinants for the association of human hormone-sensitive lipase with lipid droplets.Nature communications · 2025Article
- Induction of phospholipase A2 group 4C by HCV infection regulates lipid droplet formation.JHEP reports : innovation in hepatology · 2025Article
- Interplay between lipid droplets and alpha-synuclein: implication in Parkinson's disease pathogenesis.Frontiers in molecular neuroscience · 2025Review
- Surface tension-driven sorting of human perilipins on lipid droplets.The Journal of cell biology · 2024Article
- Roles of lipid droplets and related proteins in metabolic diseases.Lipids in health and disease · 2024Review
- Unraveling the role of lipid droplets and perilipin 2 in bovine luteal cells.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- LET-767 determines lipid droplet protein targeting and lipid homeostasis.The Journal of cell biology · 2024Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
Abstract
Perilipins (PLINs) play a key role in energy storage by orchestrating the activity of lipases on the surface of lipid droplets. Failure of this activity results in severe metabolic disease in humans. Unlike all other lipid droplet-associated proteins, PLINs localize almost exclusively to the phospholipid monolayer surrounding the droplet. To understand how they sense and associate with the unique topology of the droplet surface, we studied the localization of human PLINs inSaccharomyces cerevisiae,demonstrating that the targeting mechanism is highly conserved and that 11-mer repeat regions are sufficient for droplet targeting. Mutations designed to disrupt folding of this region into amphipathic helices (AHs) significantly decreased lipid droplet targetingin vivoandin vitro Finally, we demonstrated a substantial increase in the helicity of this region in the presence of detergent micelles, which was prevented by an AH-disrupting missense mutation. We conclude that highly conserved 11-mer repeat regions of PLINs target lipid droplets by folding into AHs on the droplet surface, thus enabling PLINs to regulate the interface between the hydrophobic lipid core and its surrounding hydrophilic environment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.