ArticlePloS one2016
Predominance of Single Prophage Carrying a CRISPR/cas System in "Candidatus Liberibacter asiaticus" Strains in Southern China.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed.
- Leaf- and canopy-level chlorotic symptom variations are associated with differentialMicrobiology spectrum · 2026Article
- Orthogroup-Based Comparative Analysis of Prophage Gene Content inInternational journal of molecular sciences · 2026Article
- Genomic footprint of a shared Type 5 prophage inApplied and environmental microbiology · 2025Article
- Molecular Characterization of 'Microorganisms · 2025Article
- Time-Course Gene Expression of 'Microorganisms · 2025Article
- Analysis of the Distribution Pattern and Prophage Types inPlants (Basel, Switzerland) · 2024Article
- The complete genome sequence of "mSphere · 2024Article
- Genetic Signatures of Contrasted Outbreak Histories of "Evolutionary applications · 2024Article
- Differential expression of "Candidatus Liberibacter solanacearum" genes and prophage loci in different life stages of potato psyllid.Scientific reports · 2024Article
- Integrated bacterial transcriptome and host metabolome analysis reveals insights into "Microbiology spectrum · 2024Article
- Pathogenicity and Transcriptomic Analyses of Two "Microbiology spectrum · 2023Article
- Huanglongbing Pandemic: Current Challenges and Emerging Management Strategies.Plants (Basel, Switzerland) · 2022Review
- Genetic Diversity of "Microbiology spectrum · 2022Article
- Characterization of the Asian Citrus Psyllid-'Microorganisms · 2022Article
- Biological Features and In Planta Transcriptomic Analyses of aInternational journal of molecular sciences · 2022Article
- An Overview of the Mechanisms Against "Frontiers in microbiology · 2022Review
- Genetic diversity and population structure of 'Candidatus Liberibacter asiaticus' associated with citrus Huanglongbing in India based on the prophage types.World journal of microbiology & biotechnology · 2021Article
- Establishment of a Cuscuta campestris-mediated enrichment system for genomic and transcriptomic analyses of 'Candidatus Liberibacter asiaticus'.Microbial biotechnology · 2021Article
- Functional and Comparative Genomic Analysis of Integrated Prophage-Like Sequences in "mSphere · 2019Article
- Citrus Genetic Engineering for Disease Resistance: Past, Present and Future.International journal of molecular sciences · 2019Review
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5 authors.
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Abstract
"Candidatus Liberibacter asiaticus" (CLas) is an uncultureable α-proteobacterium associated with citrus Huanglongbing (HLB, yellow shoot disease), a highly destructive disease affecting citrus production worldwide. HLB was observed in Guangdong Province of China over a hundred years ago and remains endemic there. Little is known about CLas biology due to its uncultureable nature. This study began with the genome sequence analysis of CLas Strain A4 from Guangdong in the prophage region. Within the two currently known prophage types, Type 1 (SC1-like) and Type 2 (SC2-like), A4 genome contained only a Type 2 prophage, CGdP2, namely. An analysis on CLas strains collected in Guangdong showed that Type 2 prophage dominated the bacterial population (82.6%, 71/86). An extended survey covering five provinces in southern China also revealed the predominance of single prophage (Type 1 or Type 2) in the CLas population (90.4%, 169/187). CLas strains with two and no prophage types accounted for 7.2% and 2.8%, respectively. In silico analyses on CGdP2 identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) system, consisting of four 22 bp repeats, three 23 bp spacers and 9 predicted cas. Similar CRISPR/cas systems were detected in all 10 published CLas prophages as well as 13 CLas field strains in southern China. Both Type 1 and Type 2 prophages shared almost identical sequences in spacer 1 and 3 but not spacer 2. Considering that the function of a CRISPR/cas system was to destroy invading DNA, it was hypothesized that a pre-established CLas prophage could use its CRISPR/cas system guided by spacer 1 and/or 3 to defeat the invasion of the other phage/prophage. This hypothesis explained the predominance of single prophage type in the CLas population in southern China. This is the first report of CRISPR/cas system in the "Ca. Liberibacter" genera.
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