ArticlePloS one2016
Discovery and Characterization of a Thermostable and Highly Halotolerant GH5 Cellulase from an Icelandic Hot Spring Isolate.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Characterization of a novel halophilic and thermostable multifunctional cellulase from Ebinur Salt Lake.Scientific reports · 2025Article
- ProteoSeeker: A Feature-Rich Metagenomic Analysis Tool for Accessible and Comprehensive Metagenomic Exploration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Biomimetic COEnvironmental science & technology · 2024Article
- Production, purification, characterization and application of two novel endoglucanases from buffalo rumen metagenome.Journal of animal science and biotechnology · 2023Article
- Review
- Expression, characterization, and activity optimization of a novel cellulase from the thermophilic bacteria Cohnella sp. A01.Scientific reports · 2022Article
- Article
- Isolation of3 Biotech · 2022Article
- In silico characterization of the GH5-cellulase family from uncultured microorganisms: physicochemical and structural studies.Journal, genetic engineering & biotechnology · 2021Article
- Current Status of Mining, Modification, and Application of Cellulases in Bioactive Substance Extraction.Current issues in molecular biology · 2021Review
- Isolation and Characterization of an Acidic, Salt-Tolerant Endoglucanase Cel5A from a Bacterial Strain Martelella endophytica YC6887 Genome.Molecular biotechnology · 2021Article
- Efficient biomass saccharification using a novel cellobiohydrolase fromRSC advances · 2021Article
- Exploring the taxonomical and functional profile of As Burgas hot spring focusing on thermostable β-galactosidases.Scientific reports · 2021Article
- High Potential for Biomass-Degrading Enzymes Revealed by Hot Spring Metagenomics.Frontiers in microbiology · 2021Article
- Thermostable Cellulases / Xylanases From Thermophilic and Hyperthermophilic Microorganisms: Current Perspective.Frontiers in bioengineering and biotechnology · 2021Review
- A novel high performance in-silico screened metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions.BMC biotechnology · 2020Article
- XynDZ5: A New Thermostable GH10 Xylanase.Frontiers in microbiology · 2020Article
- A processive endoglucanase with multi-substrate specificity is characterized from porcine gut microbiota.Scientific reports · 2019Article
- Multidisciplinary involvement and potential of thermophiles.Folia microbiologica · 2019Review
- ANASTASIA: An Automated Metagenomic Analysis Pipeline for Novel Enzyme Discovery Exploiting Next Generation Sequencing Data.Frontiers in genetics · 2019Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the ultimate goal of identifying robust cellulases for industrial biocatalytic conversions, we have isolated and characterized a new thermostable and very halotolerant GH5 cellulase. This new enzyme, termed CelDZ1, was identified by bioinformatic analysis from the genome of a polysaccharide-enrichment culture isolate, initiated from material collected from an Icelandic hot spring. Biochemical characterization of CelDZ1 revealed that it is a glycoside hydrolase with optimal activity at 70°C and pH 5.0 that exhibits good thermostability, high halotolerance at near-saturating salt concentrations, and resistance towards metal ions and other denaturing agents. X-ray crystallography of the new enzyme showed that CelDZ1 is the first reported cellulase structure that lacks the defined sugar-binding 2 subsite and revealed structural features which provide potential explanations of its biochemical characteristics.
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