Evidence map›Paper›PMID 26740620›Full record

ArticleThe Journal of biological chemistry2016

High-throughput Functional Genomics Identifies Regulators of Primary Human Beta Cell Proliferation.

Karine Robitaille, Jillian L Rourke, Joanne E McBane, Accalia Fu, Stephen Baird, Qiujiang Du, Tatsuya Kin, A M James Shapiro, Robert A Screaton

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
5.6field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 43 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Review
  9. Small-molecule discovery in the pancreatic beta cell.Current opinion in chemical biology · 2022
    Review
  10. Review
  11. Article
  12. Article
  13. Article
  14. Review
  15. Review
  16. Review
  17. Article
  18. Article
  19. The supply chain of human pancreatic β cell lines.The Journal of clinical investigation · 2019
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

Karine RobitailleFrom the Children's Hospital of Eastern Ontario Research Institute, Ottawa, Ontario K1H 8L1.
Jillian L Rourkethe Sunnybrook Research Institute, Toronto, Ontario M4N 3M5.
Joanne E McBaneFrom the Children's Hospital of Eastern Ontario Research Institute, Ottawa, Ontario K1H 8L1.
Accalia FuFrom the Children's Hospital of Eastern Ontario Research Institute, Ottawa, Ontario K1H 8L1, the Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario K1H 8M5.
Stephen BairdFrom the Children's Hospital of Eastern Ontario Research Institute, Ottawa, Ontario K1H 8L1.
Qiujiang Duthe Sunnybrook Research Institute, Toronto, Ontario M4N 3M5.
Tatsuya Kinthe Clinical Islet Transplant Program, University of Alberta, 8215-112 Street, Edmonton, Alberta T6G 2C8, and.
A M James Shapirothe Clinical Islet Transplant Program, University of Alberta, 8215-112 Street, Edmonton, Alberta T6G 2C8, and.
Robert A Screatonthe Sunnybrook Research Institute, Toronto, Ontario M4N 3M5, the Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada robert.screaton@sri.utoronto.ca.
Children's Hospital of Eastern Ontario · CASunnybrook Health Science Centre · CAUniversity of Alberta · CASunnybrook Research Institute

Funding

Canadian Institutes of Health Research MOP 111186
6 · The paper itself

Abstract

The expansion of cells for regenerative therapy will require the genetic dissection of complex regulatory mechanisms governing the proliferation of non-transformed human cells. Here, we report the development of a high-throughput RNAi screening strategy specifically for use in primary cells and demonstrate that silencing the cell cycle-dependent kinase inhibitors CDKN2C/p18 or CDKN1A/p21 facilitates cell cycle entry of quiescent adult human pancreatic beta cells. This work identifies p18 and p21 as novel targets for promoting proliferation of human beta cells and demonstrates the promise of functional genetic screens for dissecting therapeutically relevant state changes in primary human cells.

Indexed as

AdolescentAdultAgedAlbertaBiomarkersCell ProliferationCells, CulturedCyclin-Dependent Kinase Inhibitor p18Cyclin-Dependent Kinase Inhibitor p21Feasibility StudiesFemaleGenomicsGreen Fluorescent ProteinsHigh-Throughput Screening AssaysHumansInsulin-Secreting CellsBiomarkersCDKN1A protein, humanCDKN2C protein, humanCyclin-Dependent Kinase Inhibitor p18Cyclin-Dependent Kinase Inhibitor p21enhanced green fluorescent proteinGreen Fluorescent ProteinsRecombinant Fusion Proteinsbeta cell (β-cell)cell cyclehigh throughput screening (HTS)pancreatic isletprimary human cellsRNA interference (RNAi)

Identifiers

PMID26740620
PMCPMC4813485
OpenAlexW2234989637

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.