ArticleNature communications2016
Multi-reporter selection for the design of active and more specific zinc-finger nucleases for genome editing.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Who cites it
9 citing papers in PubMed, 17 citations in OpenAlex.
- High-fidelity molecular decoding through tile-guided in situ self-assembly.Science advances · 2026Article
- A Chimeric ORF Fusion Phenotypic Reporter forJournal of fungi (Basel, Switzerland) · 2024Article
- Engineered dual selection for directed evolution of SpCas9 PAM specificity.Nature communications · 2021Article
- On the prediction of DNA-binding preferences of C2H2-ZF domains using structural models: application on human CTCF.NAR genomics and bioinformatics · 2020Article
- The geometric influence on the Cys2His2 zinc finger domain and functional plasticity.Nucleic acids research · 2020Article
- Enhancing gene editing specificity by attenuating DNA cleavage kinetics.Nature biotechnology · 2019Article
- A Multireporter Bacterial 2-Hybrid Assay for the High-Throughput and Dynamic Assay of PDZ Domain-Peptide Interactions.ACS synthetic biology · 2019Article
- Linking the environment, DAF-7/TGFβ signaling and LAG-2/DSL ligand expression in the germline stem cell niche.Development (Cambridge, England) · 2017Article
- Delivery technologies for genome editing.Nature reviews. Drug discovery · 2017Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Engineered nucleases have transformed biological research and offer great therapeutic potential by enabling the straightforward modification of desired genomic sequences. While many nuclease platforms have proven functional, all can produce unanticipated off-target lesions and have difficulty discriminating between homologous sequences, limiting their therapeutic application. Here we describe a multi-reporter selection system that allows the screening of large protein libraries to uncover variants able to discriminate between sequences with substantial homology. We have used this system to identify zinc-finger nucleases that exhibit high cleavage activity (up to 60% indels) at their targets within the CCR5 and HBB genes and strong discrimination against homologous sequences within CCR2 and HBD. An unbiased screen for off-target lesions using a novel set of CCR5-targeting nucleases confirms negligible CCR2 activity and demonstrates minimal off-target activity genome wide. This system offers a straightforward approach to generate nucleases that discriminate between similar targets and provide exceptional genome-wide specificity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.