ArticleVeterinary research2016
Streptococcus uberis strains isolated from the bovine mammary gland evade immune recognition by mammary epithelial cells, but not of macrophages.
Article in Veterinary research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- Whole-Genome Sequencing Analysis Revealed High Genomic Variability, Recombination Events and Mobile Genetic Elements inAntibiotics (Basel, Switzerland) · 2025Article
- Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages.Scientific reports · 2025Article
- Endolysin NC5 improves early cloxacillin treatment in a mouse model of Streptococcus uberis mastitis.Applied microbiology and biotechnology · 2024Article
- Minthostachys verticillata essential oil modulates cytokine synthesis and Staphylococcus aureus internalization in MAC-T cells at least through TLR4/MyD88/NFkB pathway.Veterinary research communications · 2024Article
- Activation of ARP2/3 and HSP70 Expression by Lipoteichoic Acid: Potential Bidirectional Regulation of Apoptosis in a Mastitis Inflammation Model.Biomolecules · 2024Article
- Reproducible isolation of bovine mammary macrophages for analysis of host pathogen interactions.BMC veterinary research · 2024Article
- Priming from within: TLR2 dependent but receptor independent activation of the mammary macrophage inflammasome by Streptococcus uberis.Frontiers in cellular and infection microbiology · 2024Article
- Integrated analysis of inflammatory mRNAs, miRNAs, and lncRNAs elucidates the molecular interactome behind bovine mastitis.Scientific reports · 2023Article
- FABP4-mediated lipid droplet formation in Streptococcus uberis-infected macrophages supports host defence.Veterinary research · 2022Article
- Anti-inflammation of isoliquiritigenin via the inhibition of NF-κB and MAPK in LPS-stimulated MAC-T cells.BMC veterinary research · 2022Article
- A dominant clonal lineage of Streptococcus uberis in cattle in Germany.Antonie van Leeuwenhoek · 2022Article
- Progress towards the Elusive Mastitis Vaccines.Vaccines · 2022Review
- Immune defenses of the mammary gland epithelium of dairy ruminants.Frontiers in immunology · 2022Review
- Secretion of IFN-γ by Transgenic Mammary Epithelial CellsFrontiers in veterinary science · 2022Article
- Ubericin K, a New Pore-Forming Bacteriocin Targeting mannose-PTS.Microbiology spectrum · 2021Article
- Milk lymphocyte profile and macrophage functions: new insights into the immunity of the mammary gland in quarters infected with Corynebacterium bovis.BMC veterinary research · 2021Article
- Evaluation ofVaccines · 2021Article
- Diverging in vitro inflammatory responses towardJDS communications · 2021Article
- Effect ofFrontiers in veterinary science · 2021Article
- A Paradox in Bacterial Pathogenesis: Activation of the Local Macrophage Inflammasome Is Required for Virulence ofPathogens (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus uberis is frequently isolated from the mammary gland of dairy cattle. Infection with some strains can induce mild subclinical inflammation whilst others induce severe inflammation and clinical mastitis. We compared here the inflammatory response of primary cultures of bovine mammary epithelial cells (pbMEC) towards S. uberis strains collected from clinical or subclinical cases (seven strains each) of mastitis with the strong response elicited by Escherichia coli. Neither heat inactivated nor live S. uberis induced the expression of 10 key immune genes (including TNF, IL1B, IL6). The widely used virulent strain 0140J and the avirulent strain, EF20 elicited similar responses; as did mutants defective in capsule (hasA) or biofilm formation (sub0538 and sub0539). Streptococcus uberis failed to activate NF-κB in pbMEC or TLR2 in HEK293 cells, indicating that S. uberis particles did not induce any TLR-signaling in MEC. However, preparations of lipoteichoic acid (LTA) from two strains strongly induced immune gene expression and activated NF-κB in pbMEC, without the involvement of TLR2. The immune-stimulatory LTA must be arranged in the intact S. uberis such that it is unrecognizable by the relevant pathogen receptors of the MEC. The absence of immune recognition is specific for MEC, since the same S. uberis preparations strongly induced immune gene expression and NF-κB activity in the murine macrophage model cell RAW264.7. Hence, the sluggish immune response of MEC and not of professional immune cells to this pathogen may aid establishment of the often encountered belated and subclinical phenotype of S. uberis mastitis.
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