ArticleLab on a chip2016
An inverted dielectrophoretic device for analysis of attached single cell mechanics.
Article in Lab on a chip, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The trial behind it
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Who cites it
7 citing papers in PubMed.
- On-chip dielectrophoretic single-cell manipulation.Microsystems & nanoengineering · 2024Review
- Methods to mechanically perturb and characterize GUV-based minimal cell models.Computational and structural biotechnology journal · 2023Review
- Field-Controlled Microrobots Fabricated by Photopolymerization.Cyborg and bionic systems (Washington, D.C.) · 2023Review
- Article
- Single-cell mechanical analysis and tension quantification via electrodeformation relaxation.Physical review. E · 2021Article
- Cell properties assessment using optimized dielectrophoresis-based cell stretching and lumped mechanical modeling.Scientific reports · 2021Article
- Characterization of biomechanical properties of cells through dielectrophoresis-based cell stretching and actin cytoskeleton modeling.Biomedical engineering online · 2017Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Dielectrophoresis (DEP), the force induced on a polarizable body by a non-uniform electric field, has been widely used to manipulate single cells in suspension and analyze their stiffness. However, most cell types do not naturally exist in suspension but instead require attachment to the tissue extracellular matrix in vivo. Cells alter their cytoskeletal structure when they attach to a substrate, which impacts cell stiffness. It is therefore critical to be able to measure mechanical properties of cells attached to a substrate. We present a novel inverted quadrupole dielectrophoretic device capable of measuring changes in the mechanics of single cells attached to a micropatterned polyacrylamide gel. The device is positioned over a cell of defined size, a directed DEP pushing force is applied, and cell centroid displacement is dynamically measured by optical microscopy. Using this device, single endothelial cells showed greater centroid displacement in response to applied DEP pushing force following actin cytoskeleton disruption by cytochalasin D. In addition, transformed mammary epithelial cell (MCF10A-NeuT) showed greater centroid displacement in response to applied DEP pushing force compared to untransformed cells (MCF10A). DEP device measurements were confirmed by showing that the cells with greater centroid displacement also had a lower elastic modulus by atomic force microscopy. The current study demonstrates that an inverted DEP device can determine changes in single attached cell mechanics on varied substrates.
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Registered trials
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