ArticleMolecular and cellular biochemistry2016
Genome-wide analysis of tunicamycin-induced endoplasmic reticulum stress response and the protective effect of endoplasmic reticulum inhibitors in neonatal rat cardiomyocytes.
Article in Molecular and cellular biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 21 citations in OpenAlex.
- Endoplasmic reticulum stress upregulate HYOU1 and mediates lenvatinib resistance in liver cancer through ERK/MAPK pathway.Frontiers of medicine · 2025Article
- Histone deacetylase inhibition expands cellular proteostasis repertoires to enhance neuronal stress resilience.bioRxiv : the preprint server for biology · 2024Article
- Transcriptional patterns of human retinal pigment epithelial cells under protracted high glucose.Molecular biology reports · 2024Article
- Reduced Expression of Oligodendrocyte Linage-Enriched Transcripts During the Endoplasmic Reticulum Stress/Integrated Stress Response.ASN neuro · 2024Article
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- CRELD2, endoplasmic reticulum stress, and human diseases.Frontiers in endocrinology · 2023Review
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- Heteromeric Solute Carriers: Function, Structure, Pathology and Pharmacology.Advances in experimental medicine and biology · 2021Review
- Dysregulated gene-associated biomarkers for Alzheimer's disease and aging.Translational neuroscience · 2021Article
- Accurate annotation of human protein-coding small open reading frames.Nature chemical biology · 2020Article
- Utility of Extrapolating Human S1500+ Genes to the Whole Transcriptome: Tunicamycin Case Study.Bioinformatics and biology insights · 2020Article
- Mesencephalic astrocyte-derived neurotropic factor is an important factor in chondrocyte ER homeostasis.Cell stress & chaperones · 2019Article
- Expression analysis and functional characterization of the mouse cysteine-rich with EGF-like domains 2.Scientific reports · 2018Article
- ER stress and genomic instability induced by gamma radiation in mice primary cultured glial cells.Metabolic brain disease · 2018Article
- SLC3A2 is a novel endoplasmic reticulum stress-related signaling protein that regulates the unfolded protein response and apoptosis.PloS one · 2018Article
- Article
- Herpud1 negatively regulates pathological cardiac hypertrophy by inducing IP3 receptor degradation.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tunicamycin (TM) is an inducer of endoplasmic reticulum (ER) stress. However, which genes related to ER stress was induced in cardiomyocytes on a genome-wide scale remains poorly understood. Salubrinal and its derivatives are ER stress inhibitors. However, the cellular protection mechanisms remain unresolved. Neonatal rat cardiomyocytes were cultured from ventricles of one-day-old Wistar rats. Cells were exposed to salubrinal, its derivatives (PP1-12, PP1-24) or vehicle followed by TM treatment at different times. Total RNA was isolated from cells for RNA-sequencing analysis. The expressions of 189, 182, 556, 860, and 1314 genes were changed in cells exposed to TM for 1, 3, 6, 12, and 24 h. Five well-known UPR genes (Hspa5, Hsp90b1, Calr, Ddit3, and Atf4) were significantly increased in a time-dependent manner. Six not well-known genes (Hyou1, Herpud1, Manf, Creld2, Sdf2l1, and Slc3a2) were highlighted to be involved in ER stress. Compared with TM-only treated cells, the expressions of 36 genes upregulated by TM and 74 genes downregulated by TM were reversed by salubrinal. In comparison, 121 genes upregulated by TM and 92 genes downregulated by TM were reversed by PP1-12. Most genes altered by salubrinal are in the category of transcription (1 h) and cell cycle (24 h). Most genes altered by PP1-12 are in the category of response to ER stress (3 h) and cell cycle (24 h). Our findings help elucidate the mechanism for TM treatment and may be useful for future drug screens involved in ER stress.
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