ArticlePLoS neglected tropical diseases2016
Evaluation in Cameroon of a Novel, Simplified Methodology to Assist Molecular Microbiological Analysis of V. cholerae in Resource-Limited Settings.
Article in PLoS neglected tropical diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 24 citations in OpenAlex.
- Trial
- Serological responses to killed oral cholera vaccine (OCV) when given 4 years after initial receipt of OCV in Cameroon: A randomized controlled trial.PLOS global public health · 2026Article
- Review of Rapid Diagnostic Methods forThe East African health research journal · 2023Article
- TaqMan Array Card testing of participant-collected stool smears to determine the pathogen-specific epidemiology of travellers' diarrhoea†.Journal of travel medicine · 2022Article
- Spread and Endemicity of Cholera in India: Factors Beyond the Numbers.The Journal of infectious diseases · 2021Article
- Characterization of Enteric Disease in Children by Use of a Low-Cost Specimen Preservation Method.Journal of clinical microbiology · 2021Article
- Molecular characterization ofJournal of medical microbiology · 2021Article
- Three transmission events of Vibrio cholerae O1 into Lusaka, Zambia.BMC infectious diseases · 2021Article
- Wastewater based environmental surveillance of toxigenic Vibrio cholerae in Pakistan.PloS one · 2021Article
- Article
- Whole genome sequence of Vibrio cholerae directly from dried spotted filter paper.PLoS neglected tropical diseases · 2019Article
- Epidemiological and molecular forensics of cholera recurrence in Haiti.Scientific reports · 2019Article
- Whatman Protein Saver Cards for Storage and Detection of Parasitic Enteropathogens.The American journal of tropical medicine and hygiene · 2018Article
- The potential impact of case-area targeted interventions in response to cholera outbreaks: A modeling study.PLoS medicine · 2018Article
- Environmental Surveillance ofFrontiers in microbiology · 2018Article
- Direct Dried Stool Sampling on Filter Paper for Molecular Analyses of Cholera.The American journal of tropical medicine and hygiene · 2016Article
- Highlights of the 8th International Conference on Vaccines for Enteric Diseases: the Scottish Encounter To Defeat Diarrheal Diseases.Clinical and vaccine immunology : CVI · 2016Article
- Correction: Evaluation in Cameroon of a Novel, Simplified Methodology to Assist Molecular Microbiological Analysis of V. cholerae in Resource-Limited Settings.PLoS neglected tropical diseases · 2016Article
- Correction: Evaluation in Cameroon of a Novel, Simplified Methodology to Assist Molecular Microbiological Analysis of V. cholerae in Resource-Limited Settings.PLoS neglected tropical diseases · 2016Article
Corrections and comments
- Erratum issued
- Erratum issuedCorrection: Evaluation in Cameroon of a Novel, Simplified Methodology to Assist Molecular Microbiological Analysis of V. cholerae in Resource-Limited Settings.2016 · Guenou, Etiene [corrected to Guenou, Etienne]
Authors and funding
12 authors at 7 institutions in 4 countries.
Funding
Abstract
backgroundVibrio cholerae is endemic in South Asia and Africa where outbreaks of cholera occur widely and are particularly associated with poverty and poor sanitation. Knowledge of the genetic diversity of toxigenic V. cholerae isolates, particularly in Africa, remains scarce. The constraints in improving this understanding is not only the lack of regular cholera disease surveillance, but also the lack of laboratory capabilities in endemic countries to preserve, store and ship isolates in a timely manner. We evaluated the use of simplified sample preservation methods for molecular characterization using multi-locus variable-number tandem-repeat analysis (MLVA) for differentiation of Vibrio cholerae genotypes. METHODS AND
findingsForty-seven V. cholerae isolates and 18 enriched clinical specimens (e.g. stool specimens after enrichment in broth) from cholera outbreaks in Cameroon were preserved on Whatman filter paper for DNA extraction. The samples were collected from two geographically distinct outbreaks in the Far North of Cameroon (FNC) in June 2014 and October 2014. In addition, a convenience sample of 14 isolates from the Philippines and 8 from Mozambique were analyzed. All 87 DNAs were successfully analyzed including 16 paired samples, one a cultured isolate and the other the enriched specimen from which the isolate was collected. Genotypic results were identical between 15 enriched specimens and their culture isolates and the other pair differed at single locus. Two closely related, but distinct clonal complexes were identified among the Cameroonian specimens from 2014.
conclusionsCollecting V. cholerae using simplified laboratory methods in remote and low-resource settings allows for subsequent advanced molecular characterization of V. cholerae O1. These simplified DNA preservation methods identify V. cholerae and make possible timely information regarding the genetic diversity of V. cholerae; our results set the stage for continued molecular epidemiological research to better understand the transmission and dissemination of V. cholerae in Africa and elsewhere worldwide.
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