ArticlePeerJ2015
A quick and robust method for quantification of the hypersensitive response in plants.
Article in PeerJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 46 citations in OpenAlex.
- Some Like it Hot: Efficiency of the Type III Secretion System has Multiple Thermosensitive Behaviours in the Pseudomonas syringae Complex.Molecular plant pathology · 2025Article
- Programmed cell death regulator BAP2 is required for IRE1-mediated unfolded protein response in Arabidopsis.Nature communications · 2024Article
- Parallel, Continuous Monitoring and Quantification of Programmed Cell Death in Plant Tissue.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Transcriptome analysis of sugarcane reveals rapid defense response of SES208 to Xanthomonas albilineans in early infection.BMC plant biology · 2023Article
- The apple FERONIA receptor-like kinase MdMRLK2 negatively regulates Valsa canker resistance by suppressing defence responses and hypersensitive reaction.Molecular plant pathology · 2022Article
- Potentiation of plant defense by bacterial outer membrane vesicles is mediated by membrane nanodomains.The Plant cell · 2022Article
- Detection and Quantification of the Hypersensitive Response Cell Death in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Retrieving theFrontiers in microbiology · 2022Article
- HopA1 Effector fromInternational journal of molecular sciences · 2021Article
- Review
- Red light imaging for programmed cell death visualization and quantification in plant-pathogen interactions.Molecular plant pathology · 2021Article
- CATION-CHLORIDE CO-TRANSPORTER 1 (CCC1) Mediates Plant Resistance againstPlant physiology · 2020Article
- Metabolomics analysis identifies metabolites associated with systemic acquired resistance in Arabidopsis.PeerJ · 2020Article
- Active photosynthetic inhibition mediated by MPK3/MPK6 is critical to effector-triggered immunity.PLoS biology · 2018Article
- The bacterial type III-secreted protein AvrRps4 is a bipartite effector.PLoS pathogens · 2018Article
- Rhamnolipids FromFrontiers in plant science · 2018Article
- A bacterial acetyltransferase triggers immunity in Arabidopsis thaliana independent of hypersensitive response.Scientific reports · 2017Article
- ThePeerJ · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the most studied defense reactions of plants against microbial pathogens is the hypersensitive response (HR). The HR is a complex multicellular process that involves programmed cell death at the site of infection. A standard method to quantify plant defense and the HR is to measure the release of cellular electrolytes into water after infiltration with pathogenic bacteria. In this type of experiment, the bacteria are typically delivered into the plant tissue through syringe infiltration. Here we report the development of a vacuum infiltration protocol that allows multiple plant lines to be infiltrated simultaneously and assayed for defense responses. Vacuum infiltration did not induce more wounding response in Arabidopsis leaf tissue than syringe inoculation, whereas throughput and reproducibility were improved. The method was used to study HR-induced electrolyte loss after treatment with the bacterium Pseudomonas syringae pv. tomato DC3000 harboring the effector AvrRpm1, AvrRpt2 or AvrRps4. Specifically, the influence of bacterial titer on AvrRpm1-induced HR was investigated. Not only the amplitude, but also the timing of the maximum rate of the HR reaction was found to be dose-dependent. Finally, using vacuum infiltration, we were able quantify induction of phospholipase D activity after AvrRpm1 recognition in leaves labeled with (33)PO4.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.