ArticleFrontiers in genetics2015
Next-Generation Sequencing for Binary Protein-Protein Interactions.
Article in Frontiers in genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 42 citations in OpenAlex.
- Pipettes and Pipelines: The Weapons of Omics Sciences for a New Age of Clinical Studies.Current issues in molecular biology · 2026Review
- Long-read sequencing to detect full-length protein-protein interactions.Scientific reports · 2025Article
- NGPINT V3: a containerized orchestration Python software for discovery of next-generation protein-protein interactions.Bioinformatics (Oxford, England) · 2025Article
- Powdery mildew effectors AVRMolecular plant pathology · 2024Article
- Deciphering Spatial Protein-Protein Interactions in Brain Using Proximity Labeling.Molecular & cellular proteomics : MCP · 2022Review
- Unraveling Protein Interactions between the Temperate Virus Bam35 and ItsInternational journal of molecular sciences · 2021Article
- C2orf69 mutations disrupt mitochondrial function and cause a multisystem human disorder with recurring autoinflammation.The Journal of clinical investigation · 2021Article
- Identification of host factors binding to dengue and Zika virus subgenomic RNA by efficient yeast three-hybrid screens of the human ORFeome.RNA biology · 2021Article
- Next-generation yeast-two-hybrid analysis with Y2H-SCORES identifies novel interactors of the MLA immune receptor.PLoS computational biology · 2021Article
- High-resolution protein-protein interaction mapping using all-The Journal of biological chemistry · 2019Article
- Article
- DEEPN as an Approach for Batch Processing of Yeast 2-Hybrid Interactions.Cell reports · 2016Article
- Genome-Wide Transcriptional Excavation of Dipsacus asperoides Unmasked both Cryptic Asperosaponin Biosynthetic Genes and SSR Markers.Frontiers in plant science · 2016Article
- DoMY-Seq: A yeast two-hybrid-based technique for precision mapping of protein-protein interaction motifs.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The yeast two-hybrid (Y2H) system exploits host cell genetics in order to display binary protein-protein interactions (PPIs) via defined and selectable phenotypes. Numerous improvements have been made to this method, adapting the screening principle for diverse applications, including drug discovery and the scale-up for proteome wide interaction screens in human and other organisms. Here we discuss a systematic workflow and analysis scheme for screening data generated by Y2H and related assays that includes high-throughput selection procedures, readout of comprehensive results via next-generation sequencing (NGS), and the interpretation of interaction data via quantitative statistics. The novel assays and tools will serve the broader scientific community to harness the power of NGS technology to address PPI networks in health and disease. We discuss examples of how this next-generation platform can be applied to address specific questions in diverse fields of biology and medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.