Evidence map›Paper›PMID 26733730›Full record

ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2016

Effect of sample pooling and transport conditions on the clinical sensitivity of a real-time polymerase chain reaction assay for Campylobacter fetus subsp. venerealis in preputial samples from bulls.

Alvaro García-Guerra, Cheryl L Waldner, Andrea Pellegrino, Nicole Macdonald, Bonnie Chaban, Janet E Hill, Steven H Hendrick

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.3field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. A review of testing and assurance methods forFood and waterborne parasitology · 2021
    Article
  2. Article
  3. Evaluation of long-acting oxytetracycline and a commercial monovalent vaccine for the control ofThe Canadian veterinary journal = La revue veterinaire canadienne · 2017
    Article
  4. Application of direct polymerase chain reaction assays forCanadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2017
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Alvaro García-GuerraDepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (García-Guerra, Waldner, Pellegrino, Macdonald); Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (Chaban, Hill); Coaldale Veterinary Clinic, Coaldale, Alberta T1M 1M7 (Hendrick).
Cheryl L WaldnerDepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (García-Guerra, Waldner, Pellegrino, Macdonald); Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (Chaban, Hill); Coaldale Veterinary Clinic, Coaldale, Alberta T1M 1M7 (Hendrick).
Andrea PellegrinoDepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (García-Guerra, Waldner, Pellegrino, Macdonald); Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (Chaban, Hill); Coaldale Veterinary Clinic, Coaldale, Alberta T1M 1M7 (Hendrick).
Nicole MacdonaldDepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (García-Guerra, Waldner, Pellegrino, Macdonald); Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (Chaban, Hill); Coaldale Veterinary Clinic, Coaldale, Alberta T1M 1M7 (Hendrick).
Bonnie ChabanDepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (García-Guerra, Waldner, Pellegrino, Macdonald); Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (Chaban, Hill); Coaldale Veterinary Clinic, Coaldale, Alberta T1M 1M7 (Hendrick).
Janet E HillDepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (García-Guerra, Waldner, Pellegrino, Macdonald); Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (Chaban, Hill); Coaldale Veterinary Clinic, Coaldale, Alberta T1M 1M7 (Hendrick).
Steven H HendrickDepartment of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (García-Guerra, Waldner, Pellegrino, Macdonald); Department of Veterinary Microbiology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan S7N 5B4 (Chaban, Hill); Coaldale Veterinary Clinic, Coaldale, Alberta T1M 1M7 (Hendrick).
University of Saskatchewan · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The diagnosis of bovine genital campylobacteriosis (BGC) presents significant challenges, as traditional methods lack sensitivity when prolonged transport of samples is required. Assays of preputial samples by means of real-time polymerase chain reaction (PCR) provide good sensitivity and high throughput capabilities. However, there is limited information on the acceptable duration of transport and temperature during transport of samples. In addition, the use of pooled samples has proven to be a valuable strategy for the diagnosis of other venereal diseases in cattle. The objectives of the present study were to determine the effect of sample pooling and of transport time and temperature on the clinical sensitivity of a real-time quantitative PCR (qPCR) assay for Campylobacter fetus subsp. venerealis in preputial samples from beef bulls. Eight infected bulls and 176 virgin yearling bulls were used as the source of samples. The qPCR sensitivity was comparable for unpooled samples and pools of 5 samples, whereas sensitivity was decreased for pools of 10 samples. Sensitivity for the various pool sizes improved with repeated sampling. For shorter-term transport (2 and 48 h), sensitivity was greatest when the samples were stored at 4°C and 30°C, whereas for longer-term transport (96 h) sensitivity was greatest when the samples were stored at -20°C. The creation of pools of 5 samples is therefore a good option to decrease costs when screening bulls for BGC with the qPCR assay of direct preputial samples. Ideally the samples should be stored at 4°C and arrive at the laboratory within 48 h of collection, but when that is not possible freezing at -20°C could minimize the loss of sensitivity.

Indexed as

AnimalsCampylobacter fetusCampylobacter InfectionsCattleCattle DiseasesForeskinMaleReal-Time Polymerase Chain ReactionSexually Transmitted Diseases, BacterialSpecimen Handling

Identifiers

PMID26733730
PMCPMC4686032
OpenAlexW2416233740

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.