ArticleCanadian journal of veterinary research = Revue canadienne de recherche veterinaire2016
Ring test evaluation of the detection of influenza A virus in swine oral fluids by real-time reverse-transcription polymerase chain reaction and virus isolation.
Abstract read
In one paragraph
Article in Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
0numbers the graph read from it
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10citing papers in PubMed
–field-weighted citation impact
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Who cites it
10 citing papers in PubMed.
- Article
- Influenza surveillance in pigs: balancing act between broad diagnostic coverage and specific virus characterization.Porcine health management · 2024Article
- Article
- Normalizing real-time PCR results in routine testing.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- Effect of extrinsic factors on the detection of PRRSV and a porcine-specific internal sample control in serum, oral fluid, and fecal specimens tested by RT-rtPCR.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023Article
- Temporal Patterns of Phenotypic Antimicrobial Resistance and Coinfecting Pathogens inPathogens (Basel, Switzerland) · 2022Article
- Coinfections and Phenotypic Antimicrobial Resistance inFrontiers in veterinary science · 2021Article
- Guidelines for oral fluid-based surveillance of viral pathogens in swine.Porcine health management · 2020Review
- Article
- Influenza Herd-Level Prevalence and Seasonality in Breed-to-Wean Pig Farms in the Midwestern United States.Frontiers in veterinary science · 2017Article
Corrections and comments
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Authors and funding
25 authors.
Christa K GoodellDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Jianqiang ZhangDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Erin StraitDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Karen HarmonDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Devi PatnayakDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Tracy OttersonDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Marie CulhaneDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Jane Christopher-HenningsDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Travis ClementDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Pamela Leslie-SteenDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Richard HesseDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Joe AndersonDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Kevin SkarbekDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Amy VincentDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Pravina KitikoonDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Sabrina SwensonDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Melinda Jenkins-MooreDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Jodi McGillDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Rolf RauhDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
William NelsonDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Catherine O'ConnellDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Rohan ShahDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Chong WangDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Rodger MainDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Jeffrey J ZimmermanDepartment of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, Iowa 50011, USA, and Veterinary Medical Research Institute (Building 1), 1802 University Boulevard, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011-1240, USA (Goodell, Zhang, Strait, Harmon, Wang, Main, Zimmerman); Department of Statistics, Iowa State University, Ames, Iowa 50011, USA (Wang); Minnesota Veterinary Diagnostic Laboratory, University of Minnesota, St. Paul, Minnesota 55108, USA (Patnayak, Otterson, Culhane); Animal Disease Research and Diagnostic Laboratory, South Dakota State University, Brookings, South Dakota 57007, USA (Christopher-Hennings, Clement, Leslie-Steen); Kansas State Veterinary Diagnostic Laboratory, Kansas State University, Manhattan, Kansas 66506, USA (Hesse, Anderson, Skarbek); US Department of Agriculture (USDA) National Animal Disease Center, Ames, Iowa 50010, USA (Vincent, Kitikoon, McGill); USDA National Veterinary Services Laboratories, Ames, Iowa 50010, USA (Swenson, Jenkins-Moore); Tetracore, Rockville, Maryland, 20850, USA (Rauh, Nelson); Thermo Fisher Scientific (Life Technologies), Austin, Texas 78744, USA (O'Connell, Shah).
Funding
No grant is acknowledged in the PubMed record.
Abstract
The probability of detecting influenza A virus (IAV) in oral fluid (OF) specimens was calculated for each of 13 assays based on real-time reverse-transcription polymerase chain reaction (rRT-PCR) and 7 assays based on virus isolation (VI). The OF specimens were inoculated with H1N1 or H3N2 IAV and serially diluted 10-fold (10(-1) to 10(-8)). Eight participating laboratories received 180 randomized OF samples (10 replicates × 8 dilutions × 2 IAV subtypes plus 20 IAV-negative samples) and performed the rRT-PCR and VI procedure(s) of their choice. Analysis of the results with a mixed-effect logistic-regression model identified dilution and assay as variables significant (P < 0.0001) for IAV detection in OF by rRT-PCR or VI. Virus subtype was not significant for IAV detection by either rRT-PCR (P = 0.457) or VI (P = 0.101). For rRT-PCR the cycle threshold (Ct) values increased consistently with dilution but varied widely. Therefore, it was not possible to predict VI success on the basis of Ct values. The success of VI was inversely related to the dilution of the sample; the assay was generally unsuccessful at lower virus concentrations. Successful swine health monitoring and disease surveillance require assays with consistent performance, but significant differences in reproducibility were observed among the assays evaluated.
Indexed as
Influenza A Virus, H1N1 SubtypeInfluenza A Virus, H3N2 SubtypeAnimalsFemaleOrthomyxoviridae InfectionsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSalivaSwineSwine Diseases
Identifiers
PMID
26733728PMC
PMC4686030What OpenQuestion holds
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Read underepoch 390
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