ArticleProceedings of the National Academy of Sciences of the United States of America2016
Bacterial RNA polymerase can retain σ70 throughout transcription.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 76 citations in OpenAlex.
- Actuation of CRP activating region 3 by formylation modulates theProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Structural basis for multi-subunit DNA-dependent RNA polymerase catalytic activity.Molecular cell · 2026Article
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- Structure-to-function relationships of RNA polymerases: recent advances in bacterial transcription termination.Transcription · 2026Review
- Structural basis of pausing during transcription initiation in mycobacterium tuberculosis.Nature communications · 2026Article
- Single-molecule experiments reveal NusG displaces transcription initiation factor σ70 from mature elongation complexes.Nucleic acids research · 2026Article
- Differential association of primary and alternative σ factors with RNA polymerase of Mycobacterium tuberculosis during transcription elongation.Nucleic acids research · 2026Article
- Structural Insights into De Novo Promoter Escape by Mycobacterium tuberculosis RNA Polymerase.Nature communications · 2025Article
- Sticking around: σ factor dynamics and implications for transcription elongation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- How do bacteria tune transcription termination efficiency?Current opinion in microbiology · 2024Review
- Force and the α-C-terminal domains bias RNA polymerase recycling.Nature communications · 2024Article
- Article
- Structural basis of σProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Recycling of bacterial RNA polymerase by the Swi2/Snf2 ATPase RapA.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Transcriptional activators in the early Drosophila embryo perform different kinetic roles.Cell systems · 2023Article
- Head-on and co-directional RNA polymerase collisions orchestrate bidirectional transcription termination.Molecular cell · 2023Article
- Recycling of Bacterial RNA Polymerase by the Swi2/Snf2 ATPase RapA.bioRxiv : the preprint server for biology · 2023Article
- Principles of gene regulation quantitatively connect DNA to RNA and proteins in bacteria.Science (New York, N.Y.) · 2022Article
- Structural advances in transcription elongation.Current opinion in structural biology · 2022Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Production of a messenger RNA proceeds through sequential stages of transcription initiation and transcript elongation and termination. During each of these stages, RNA polymerase (RNAP) function is regulated by RNAP-associated protein factors. In bacteria, RNAP-associated σ factors are strictly required for promoter recognition and have historically been regarded as dedicated initiation factors. However, the primary σ factor in Escherichia coli, σ(70), can remain associated with RNAP during the transition from initiation to elongation, influencing events that occur after initiation. Quantitative studies on the extent of σ(70) retention have been limited to complexes halted during early elongation. Here, we used multiwavelength single-molecule fluorescence-colocalization microscopy to observe the σ(70)-RNAP complex during initiation from the λ PR' promoter and throughout the elongation of a long (>2,000-nt) transcript. Our results provide direct measurements of the fraction of actively transcribing complexes with bound σ(70) and the kinetics of σ(70) release from actively transcribing complexes. σ(70) release from mature elongation complexes was slow (0.0038 s(-1)); a substantial subpopulation of elongation complexes retained σ(70) throughout transcript elongation, and this fraction depended on the sequence of the initially transcribed region. We also show that elongation complexes containing σ(70) manifest enhanced recognition of a promoter-like pause element positioned hundreds of nucleotides downstream of the promoter. Together, the results provide a quantitative framework for understanding the postinitiation roles of σ(70) during transcription.
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