Evidence map›Paper›PMID 26732502›Full record

ArticleEuropean journal of nutrition2017

Fenugreek increases insulin-stimulated creatine content in L6C11 muscle myotubes.

Kristyen A Tomcik, William J Smiles, Donny M Camera, Helmut M Hügel, John A Hawley, Rani Watts

Abstract read
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In one paragraph

Article in European journal of nutrition, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it, 13 citations in OpenAlex.

  1. Pooled it
  2. Efficacy of FurosapInternational journal of medical sciences · 2017
    Trial
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Kristyen A TomcikCentre for Exercise and Nutrition, Mary MacKillop Institute for Health Research, Australian Catholic University, Melbourne, VIC, 3065, Australia.
William J SmilesCentre for Exercise and Nutrition, Mary MacKillop Institute for Health Research, Australian Catholic University, Melbourne, VIC, 3065, Australia.
Donny M CameraCentre for Exercise and Nutrition, Mary MacKillop Institute for Health Research, Australian Catholic University, Melbourne, VIC, 3065, Australia.
Helmut M HügelSchool of Applied Sciences and Health Innovations Research Institute, RMIT University, Melbourne, VIC, Australia.
John A HawleyCentre for Exercise and Nutrition, Mary MacKillop Institute for Health Research, Australian Catholic University, Melbourne, VIC, 3065, Australia. john.hawley@acu.edu.au.
Rani WattsCentre for Exercise and Nutrition, Mary MacKillop Institute for Health Research, Australian Catholic University, Melbourne, VIC, 3065, Australia.
Australian Catholic University · AURMIT University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeCreatine uptake by muscle cells is increased in the presence of insulin. Accordingly, compounds with insulin-like actions may also augment creatine uptake. The aim of this study was to investigate whether Trigonella foenum-graecum (fenugreek), an insulin mimetic, increases total intracellular creatine levels in vitro.

methodsTotal cellular creatine content was measured fluorometrically in L6C11 muscle myotubes treated for 1, 4, and 24 h with 0.5 mM creatine (CR), CR and 20 μg/mL fenugreek seed extract (CR + FEN), CR and 100 nM insulin (CR + INS), and CR + INS + FEN (n = 6 per treatment group). Alterations in the expression of the sodium- and chloride-dependent creatine transporter, SLC6A8, and key signaling proteins in the PI3-K/Akt pathway were determined.

resultsCompared to control (CON), CR + INS + FEN increased total creatine content after 4 h (P < 0.05), whereas all conditions increased SLC6A8 protein expression above CON at this time (P < 0.05). Changes in insulin signaling were demonstrated via increases in Akt

conclusionFenugreek, when combined with insulin, modulates creatine content via a mechanism which is independent of the activity of SLC6A8, suggesting that an alternative mechanism is responsible for the regulation and facilitation of insulin-mediated creatine uptake in skeletal muscle cells.

Indexed as

AnimalsCell LineCell SurvivalCreatineGene Expression RegulationGlucose Transporter Type 4Hypoglycemic AgentsInsulinMuscle Fibers, SkeletalNerve Tissue ProteinsPhosphatidylinositol 3-KinasesPhosphorylationPlant ExtractsPlasma Membrane Neurotransmitter Transport ProteinsRatsSignal TransductionCreatinefenugreek seed mealGlucose Transporter Type 4Hypoglycemic AgentsInsulinNerve Tissue ProteinsPhosphatidylinositol 3-KinasesPlant ExtractsPlasma Membrane Neurotransmitter Transport ProteinsSlc2a4 protein, ratSlc6a8 protein, ratCreatine contentCreatine transportFenugreek supplementInsulin signaling

Identifiers

PMID26732502
OpenAlexW2225233910

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.