ArticlePloS one2016
Validation of a Cariogenic Biofilm Model to Evaluate the Effect of Fluoride on Enamel and Root Dentine Demineralization.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 69 citations in OpenAlex.
- Biofilm extracellular polysaccharides degradation during starvation and enamel demineralization.PloS one · 2017Trial
- In-vitro effect of a single application of CPP-ACP pastes and different fluoridated solutions on the prevention of dental caries around orthodontic brackets.Dental press journal of orthodontics · 2024Article
- Er,Cr:YSGG and 980nm diode lasers influence dentin surface volume after cariogenic challenge: in vitro study.Brazilian oral research · 2024Article
- In Vitro Models Used in the Formation of Root Caries Lesions-A Review of the Literature.Dentistry journal · 2023Review
- Developing Bioactive Dental Resins for Restorative Dentistry.Journal of dental research · 2023Review
- Acidic Microenvironment-Sensitive Core-Shell Microcubes: The Self-assembled and the Therapeutic Effects for Caries Prevention.European journal of dentistry · 2023Article
- Smart dental materials for antimicrobial applications.Bioactive materials · 2023Article
- Engineered Salivary Peptides Reduce Enamel Demineralization Provoked by CariogenicMicroorganisms · 2022Article
- The influence of biofilm maturation on fluoride's anticaries efficacy.Clinical oral investigations · 2022Article
- In vitro model systems for exploring oral biofilms: From single-species populations to complex multi-species communities.Journal of applied microbiology · 2022Review
- In-vitro evaluation of the anti-cariogenic effect of a hybrid coating associated with encapsulated sodium fluoride and stannous chloride in nanoclays on enamel.Journal of applied oral science : revista FOB · 2022Article
- In Silico Modeling of Hyposalivation and Biofilm Dysbiosis in Root Caries.Journal of dental research · 2021Article
- Functionalized Mouth-Conformable Interfaces for pH Evaluation of the Oral Cavity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Effect of ionizing radiation and cariogenic biofilm challenge on root-dentin caries.Clinical oral investigations · 2021Article
- Root Caries Preventive Effect of Varnishes Containing Fluoride or Fluoride + Chlorhexidine/Cetylpyridinium Chloride In Vitro.Microorganisms · 2021Article
- Synthesis of a chitosan nanoparticle suspension and its protective effects against enamel demineralization after an in vitro cariogenic challenge.Journal of applied oral science : revista FOB · 2021Article
- Commercial Silver Diamine Fluoride (SDF) Products on Caries Lesion Progression in Primary Enamel: An In Vitro Study.Oral health & preventive dentistry · 2020Article
- Effect of Etching Procedures on the Adhesion of Biofilm-Coated Dentin.Materials (Basel, Switzerland) · 2020Article
- Remineralization of Artificial Dentin Caries Using Dentin and Enamel Matrix Proteins.Materials (Basel, Switzerland) · 2019Article
- A Multispecies Biofilm In Vitro Screening Model of Dental Caries for High-Throughput Susceptibility Testing.High-throughput · 2019Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to gingival recession both enamel and root dentine are at risk of developing caries. Both tissues are exposed to a similar environment, however there is not a validated model to evaluate the effect of fluoride on these dental substrates simultaneously. Hence, this study aimed to validate a caries model to evaluate the effect of fluoride to prevent demineralization on enamel and root-dentine. Streptococcus mutans UA159 biofilms were formed on saliva-coated bovine enamel and root dentine slabs (n = 12 per group) mounted in the same well of culture plates. The biofilms were exposed 8×/day to 10% sucrose and treated 2×/day with fluoridated solutions containing 0, 150, 450, or 1,350 ppm F; thus, simulating the use of low to high fluoride concentration toothpastes. The pH values of the culture medium was monitored 2×/day as a biofilm acidogenicity indicator. After 96 h, biofilms were collected for fluoride concentration analysis. The percentage of surface hardness loss (%SHL) was calculated for slabs. The fluoride uptake by the enamel and dentine was also determined. The model showed a dose-response because the biofilm and fluoride uptake increased and %SHL decreased at increasing fluoride concentrations (p < 0.05). Fluoride in the biofilm formed on dentine and fluoride uptake by dentine were higher than those for enamel. With the same fluoride concentration treatment, the percentage of reduction of demineralization was lower for dentine than for enamel. In conclusion, the model was validated in terms of a dose-response effect of fluoride on enamel and root dentine. Furthermore, the findings support the clinical data, suggesting that higher fluoride concentrations are necessary to control caries of root dentine than of enamel.
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