ArticlePloS one2016
Nanoscale Imaging Reveals a Tetraspanin-CD9 Coordinated Elevation of Endothelial ICAM-1 Clusters.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- The potassium channel KNature communications · 2025Article
- Human tetraspanin CD81 facilitates invasion ofVirulence · 2024Article
- Quantitative imaging of vesicle-protein interactions reveals close cooperation among proteins.Journal of extracellular vesicles · 2023Article
- T cell-dependent bispecific antibodies alter organ-specific endothelial cell-T cell interaction.EMBO reports · 2023Article
- Rac1 regulates lipid droplets formation, nanomechanical, and nanostructural changes induced by TNF in vascular endothelium in the isolated murine aorta.Cellular and molecular life sciences : CMLS · 2022Article
- How cell migration helps immune sentinels.Frontiers in cell and developmental biology · 2022Review
- Lipid Droplets Formation Represents an Integral Component of Endothelial Inflammation Induced by LPS.Cells · 2021Article
- CD9, a tetraspanin target for cancer therapy?Experimental biology and medicine (Maywood, N.J.) · 2021Review
- Intravascular Crawling of Patrolling Monocytes: A Lèvy-Like Motility for Unique Search Functions?Frontiers in immunology · 2021Review
- Elucidating the Biomechanics of Leukocyte Transendothelial Migration by Quantitative Imaging.Frontiers in cell and developmental biology · 2021Review
- Molecular mechanisms of dendritic cell migration in immunity and cancer.Medical microbiology and immunology · 2020Review
- Effects of Tumor Necrosis Factor α (TNF-α) and Interleukina 10 (IL-10) on Intercellular Cell Adhesion Molecule-1 (ICAM-1) and Cluster of Differentiation 31 (CD31) in Human Coronary Artery Endothelial Cells.Medical science monitor : international medical journal of experimental and clinical research · 2018Article
- Tetraspanin CD9: A Key Regulator of Cell Adhesion in the Immune System.Frontiers in immunology · 2018Review
- The Many and Varied Roles of Tetraspanins in Immune Cell Recruitment and Migration.Frontiers in immunology · 2018Review
- Characterisation of Plasmodium falciparum populations selected on the human endothelial receptors P-selectin, E-selectin, CD9 and CD151.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endothelial barriers have a central role in inflammation as they allow or deny the passage of leukocytes from the vasculature into the tissue. To bind leukocytes, endothelial cells form adhesive clusters containing tetraspanins and ICAM-1, so-called endothelial adhesive platforms (EAPs). Upon leukocyte binding, EAPs evolve into docking structures that emanate from the endothelial surface while engulfing the leukocyte. Here, we show that TNF-α is sufficient to induce apical protrusions in the absence of leukocytes. Using advanced quantitation of atomic force microscopy (AFM) recordings, we found these structures to protrude by 160 ± 80 nm above endothelial surface level. Confocal immunofluorescence microscopy proved them positive for ICAM-1, JAM-A, tetraspanin CD9 and f-actin. Microvilli formation was inhibited in the absence of CD9. Our findings indicate that stimulation with TNF-α induces nanoscale changes in endothelial surface architecture and that--via a tetraspanin CD9 depending mechanism--the EAPs rise above the surface to facilitate leukocyte capture.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.